AB177828

Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)]

RabMAb
Advanced Validation
Recombinant
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Rabbit Recombinant Monoclonal Histone H4 mono methyl K77 antibody. Suitable for WB, PepArr and reacts with Mouse, Human samples. Cited in 1 publication.

대체 명칭 보기

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K77me, H4K77me1, H4K77

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Supplier Data

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution

All lanes:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 1min

Peptide Array - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

PepArr

Supplier Data

Peptide Array - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

ab177828 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17921(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

PepArr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 meK77

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer discovery : PubMed40997327

2025

Functional mapping of epigenomic regulators uncovers coordinated tumor suppression by the HBO1 and MLL1 complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuning J Tang,Haiqing Xu,Nicholas W Hughes,Paloma Ruiz,Samuel H Kim,Emily G Shuldiner,Steven S Lopez,Jess D Hebert,Saswati Karmakar,Laura Andrejka,Deniz Nesli Dolcen,Gabor Boross,Pauline Chu,Christian A Kunder,Colin Detrick,Sarah E Pierce,Emily L Ashkin,William J Greenleaf,Anne K Voss,Tim Thomas,Matt van de Rijn,Dmitri A Petrov,Monte M Winslow

제품이 사용된 논문 모두 보기

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peptideArrayWebsite

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)]

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

Peptide Array - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (AB177828)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise