Anti-HIV1 p55 + p24 antibody [EPR27327-79]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HIV1 p55 + p24 with ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human cerebrum. The section was incubated with ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling HIV1 p55 + p24 with ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HIV1 p55 + p24 with ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Immunohistochemical analysis of paraffin-embedded cells labeling HIV1 p55 + p24 with ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a HIV1-GAG His tag construct, no staining on (B) HEK-293T transfected with a HIV2-GAG His tag construct and (C) HEK-293T transfected with empty plasmid. The section was incubated with ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling HIV1 p55 + p24 with ab308072 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a HIV1-GAG expression vector containing a myc tag and no staining in HEK-293T cells transfected with a HIV2-GAG expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged HIV2-GAG overexpression construct (Left) / HEK-293T cells transfected with Myc-tagged HIV1-GAG overexpression construct (Right) labelling HIV1 p55 + p24 with ab308072 at 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-myc conjugated to 647.

• I-ELISA

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Indirect ELISA - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Indirect ELISA analysis of ab308072 at 0-1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

• WB

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Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (ab308072) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a HIV-GAG p55 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (ab308072) at 1/1000 dilution

Lane 1:

His-tagged HIV2-GAG recombinant protein at 10 ng

Lane 2:

His-tagged HIV1-GAG p17 fragment at 10 ng

Lane 3:

His-tagged HIV1-GAG p24 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 24 kDa

false

Exposure time: 3s