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AB248295

Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free

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Rabbit Recombinant Monoclonal HMBS/PBGD antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
2 이미지
Western blot - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (AB248295)
  • WB

Unknown

Western blot - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (AB248295)

This data was developed using ab129092, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HMBS/PBGD antibody [EPR8105] (<a href='/ko/products/primary-antibodies/hmbs-pbgd-antibody-epr8105-ab129092'>ab129092</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

U937 cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 42-44 kDa

false

OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (AB248295)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (AB248295)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8105

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Sample Prep & Detection Kits

AB269822

Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (AB269822)

  • 5
  • 1
Sample Prep & Detection Kits

AB269903

Gold-Maleimide Conjugation Kit (10nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (10nm, 20 OD) (AB269903)

  • 0
  • 0
Primary Antibodies

AB207732

Anti-BCRP/ABCG2 antibody [EPR20080]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)

  • 4
  • 6
Primary Antibodies

AB137042

Anti-FECH antibody [EPR8312]

RabMAb|Recombinant

Western blot - Anti-FECH antibody [EPR8312] (AB137042)

  • 0
  • 0
Primary Antibodies

AB154860

Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860)

  • 5
  • 2
Assay Kits

AB228554

Cell Counting Kit 8 (WST-8 / CCK8)

Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (AB228554)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

ab248295 is the carrier-free version of ab129092.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen (PubMed : 18004775, PubMed : 18936296, PubMed : 19138865, PubMed : 23815679). Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled (PubMed : 18936296). In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact (PubMed : 18936296).
See full target information HMBS

대체 명칭 보기

PBGD, UPS, HMBS, Porphobilinogen deaminase, PBG-D, Hydroxymethylbilane synthase, Pre-uroporphyrinogen synthase
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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