Anti-HMGA2 antibody [EPR29217-93]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Immunohistochemical analysis of paraffin-embedded Rat E12.5 tissue labeling HMGA2 with ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat E12.5.
The section was incubated with ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Immunohistochemical analysis of paraffin-embedded Mouse E12.5 tissue labeling HMGA2 with ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse E12.5 (PMID : 7651535).
The section was incubated with ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling HMGA2 with ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat liver.
The section was incubated with ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HMGA2 with ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on mouse liver.
The section was incubated with ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 3 sample was prepared via the 1% SDS HOT lysis method, lane 1 and lane 2 samples were prepared via RIPA lysis.

The identity of the higher MW band at approximately 37 kDa (in lane 3) is unknown.

All lanes:

Western blot - Anti-HMGA2 antibody [EPR29217-93] (ab319057) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole (RIPA) cell lysate at 20 µg

Lane 2:

NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole (RIPA) cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole (HOT) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PIMD : 7651535)

Lane 1 and lane 2 samples were prepared via the 1% SDS HOT lysis method(100℃, 30 min), lane 3 and lane 5 were prepared via RIPA lysis.

The lysate of lane 5 was freshly made and used immediately to minimize protein degradation.

The identity of the higher MW band at approximately 37 kDa (in lane 4) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HMGA2 antibody [EPR29217-93] (ab319057) at 1/1000 dilution

Lane 1:

Mouse E12 embryo tissue (HOT) lysate at 20 µg

Lane 2:

Mouse liver tissue (HOT) lysate at 20 µg

Lane 3:

Mouse E12 embryo tissue (RIPA) lysate at 20 µg

Lane 4:

Mouse liver tissue (RIPA) lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole (RIPA) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody [EPR29217-93] (AB319057)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HMGA1.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-HMGA2 antibody [EPR29217-93] (ab319057) at 1/1000 dilution

Lane 1:

His tagged human HMGA2 fragment at 10 ng

Lane 2:

His tagged human HMGA1 fragment at 40 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa

false

Exposure time: 26s