AB215437

Anti-HMGCR (phospho S872) antibody

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Rabbit Polyclonal HMGCR phospho S872 antibody. Suitable for ELISA, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HMGCR pS872 aa 800 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for HMGCR using ab215437 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

Formalin-fixed, paraffin-embedded mouse intestine tissue stained for HMGCR using ab215437 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

Formalin-fixed, paraffin-embedded rat liver tissue stained for HMGCR using ab215437 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

ELISA - Anti-HMGCR (phospho S872) antibody (AB215437)

ELISA

Supplier Data

ELISA - Anti-HMGCR (phospho S872) antibody (AB215437)

ELISA was performed using a serial dilution of ab215437 in antigen-coated wells. The antigen used was a peptide containing the phosphosite of interest. The plot shows the absorbance against the antibody dilution for phosphopeptide and non phosphopeptide antigen.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Human HMGCR pS872 aa 800 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04035

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/500 - 1/1000", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HMG-CoA reductase also known as HMGCR plays an important role in cholesterol biosynthesis. This enzyme catalyzes the conversion of HMG-CoA (3-hydroxy-3-methyl-glutaryl-coenzyme A) to mevalonate a critical step in the isoprenoid synthesis pathway. HMGCR is a membrane-bound enzyme located primarily in the liver on the endoplasmic reticulum. The enzyme has a molecular mass of approximately 97 kDa. Due to its central role in cholesterol metabolism HMGCR has become a primary target for statins such as pravastatin and simvastatin.

Biological function summary

HMGCR reduces HMG-CoA to mevalonate an important precursor for several important biomolecules including cholesterol. It exists as part of a complex regulatory system impacted by intracellular cholesterol levels. The activity of HMGCR is tightly controlled through feedback inhibition and post-translational modifications. The enzyme interacts with other proteins in the endoplasmic reticulum as part of the cholesterol homeostatic mechanism in cells expressing it including hepatocytes.

Pathways

HMGCR is an essential component of the mevalonate pathway which is important for the synthesis of sterols and non-sterol isoprenoids. This pathway also intersects with the biosynthesis of ubiquinone and dolichol. The regulation of cholesterol synthesis involves the coordinated activity of HMG-CoA reductase and LDL receptor proteins. Their interplay ensures a balanced cholesterol level and integrates with the response to cellular demands and dietary intake.

HMGCR is strongly linked to cardiovascular diseases due to its influential role in cholesterol synthesis. Inhibitors like pravastatin and simvastatin effectively lower blood cholesterol levels and reduce the risk of coronary artery disease by targeting HMGCR. The Statins also have potential associations with muscular disorders because of shared pathways involving proteins such as SREBP (sterol regulatory element-binding proteins) which regulate the transcription of genes involved in lipid metabolism.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed : 21357570, PubMed : 2991281, PubMed : 36745799, PubMed : 6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed : 11349148, PubMed : 18540668, PubMed : 36745799).

See full target information HMGCR pS872

대체 명칭 보기

3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase, HMGCR

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of experimental & clinical cancer research : CR 44:112 PubMed40181456

2025

TRAF7 knockdown induces cellular senescence and synergizes with lomustine to inhibit glioma progression and recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Tongyu Zhou,Rongrong Zhou,Wen Sun,Yan Li,Qiyi Zhou,Dongcheng Xu,Yuxin Zhao,Peihao Hu,Jingrui Liang,Yumeng Zhang,Bin Zhong,Juncheng Yao,Di Jing

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMGCR (phospho S872) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR (phospho S872) antibody (AB215437)

ELISA - Anti-HMGCR (phospho S872) antibody (AB215437)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise