AB236667

Anti-HMGCS2 antibody

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Rabbit Polyclonal HMGCS2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Hydroxymethylglutaryl-CoA synthase, mitochondrial aa 400 to C-terminus.

대체 명칭 보기

HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS2

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody (AB236667)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody (AB236667)

Paraffin-embedded human liver cancer tissue stained for HMGCS2 using ab236667 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-HMGCS2 antibody (AB236667)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGCS2 antibody (AB236667)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for HMGCS2 using ab236667 at 1/133 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody (AB236667)

Paraffin-embedded human liver tissue stained for HMGCS2 using ab236667 at 1/400 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Hydroxymethylglutaryl-CoA synthase, mitochondrial aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54868

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HMGCS2 also known as hydroxymethylglutaryl-CoA synthase 2 is an enzyme involved in ketogenesis. It catalyzes the conversion of acetyl-CoA to HMG-CoA an essential step in the production of ketone bodies. This enzyme has a molecular mass of approximately 57 kDa. HMGCS2 is mainly expressed in the liver and is located within the mitochondria where it works under controlled metabolic conditions.

Biological function summary

HMGCS2 activates the ketogenesis process which generates ketone bodies such as acetoacetate and β-hydroxybutyrate that serve as alternative energy sources when glucose is scarce. This enzyme is not known to be part of any larger protein complex. During fasting states or prolonged exercise the liver uptakes fatty acids and converts them into ketone bodies through HMGCS2 activity providing energy especially for organs like the brain and muscles.

Pathways

HMGCS2 functions in the ketogenesis and lipid metabolism pathways. It works alongside enzymes like HMG-CoA lyase which further processes HMG-CoA into acetoacetate a precursor for other ketone bodies. The interplay of HMGCS2 with fatty acid oxidation allows it to regulate energy homeostasis coordinating with enzymes such as carnitine palmitoyltransferase I which aids in transporting fatty acids into mitochondria for oxidation.

HMGCS2 malfunctions or deficiencies relate to disorders such as hypoketotic hypoglycemia and hepatic encephalopathy. Disrupted activity can affect energy balance particularly during fasting impacting glucose homeostasis. Alterations in HMGCS2 expression or function can connect with proteins like medium-chain acyl-CoA dehydrogenase which plays roles in fatty acid metabolism disorders further complicating the metabolic landscape when HMGCS2 is impaired.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.

See full target information Hydroxymethylglutaryl-CoA synthase, mitochondrial

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cell research & therapy 15:492 PubMed39707501

2024

FDFT1 maintains glioblastoma stem cells through activation of the Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Mo,Jiajia Shao,Zhun Li,Peiting Zeng,Xinke Yin,Yongsheng Huang,Peng Wang,Jianwei Liao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMGCS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody (AB236667)

Immunocytochemistry/ Immunofluorescence - Anti-HMGCS2 antibody (AB236667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody (AB236667)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise