AB236759

Anti-HNF-1B antibody [CL0374]

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(5 제품이 사용된 논문 )

Mouse Monoclonal HNF-1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1B aa 1-150.

대체 명칭 보기

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

7 이미지

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling HNF-1B (green) using ab236759 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human tonsil tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis. Negative control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human renal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colorectal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colon tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human kidney tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

All lanes:

Western blot - Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/mL

Lane 1:

Negative control, vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HNF-1B over-expression (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells) cell lysate

Predicted band size: 61 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0374

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HNF1B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P35680

Epitope

Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides.

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대체품

Primary Antibodies

AB128912

Anti-HNF-1B antibody [EPR6334(2)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Primary Antibodies

AB213149

Anti-HNF-1B antibody [EPR18644-13]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (AB213149)

Primary Antibodies

AB213150

Anti-HNF-1B antibody [EPR18644-37]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] (AB213150)

Primary Antibodies

AB238980

Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)

보완 제품

Assay Kits

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Glucose Uptake Assay Kit (Colorimetric)

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Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

Primary Antibodies

AB227707

Anti-PAX8 antibody [SP348] - N-terminal

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Multiplex immunohistochemistry - Anti-PAX8 antibody [SP348] - N-terminal (AB227707)

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

Primary Antibodies

AB185966

Anti-SOX9 antibody [EPR14335-78]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (AB185966)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.

Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).

See full target information HNF1B

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Lupus science & medicine 11: PubMed39608815

2024

HNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Mei Zou,Jie Yu,Yuan-Yuan Ruan,Ying Xie,Xiao-Min An,Pei-Lei Chen,Ying-Qin Luo,Ming-Jun Shi,Miao Liu,Li-Fen Xu,Jun Liu,Bing Guo,Fan Zhang

JCI insight 7: PubMed34874920

2022

Angiotensin-(1-7)/MasR axis promotes migration of monocytes/macrophages with a regulatory phenotype to perform phagocytosis and efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Zaidan,Luciana P Tavares,Michelle A Sugimoto,Kátia M Lima,Graziele L Negreiros-Lima,Lívia Cr Teixeira,Thais C Miranda,Bruno Vs Valiate,Allysson Cramer,Juliana Priscila Vago,Gabriel H Campolina-Silva,Jéssica Am Souza,Laís C Grossi,Vanessa Pinho,Maria Jose Campagnole-Santos,Robson As Santos,Mauro M Teixeira,Izabela Galvão,Lirlândia P Sousa

Oncology letters 23:9 PubMed34820008

2021

Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Watabe,Sayaka Kobayashi,Mizuho Hatori,Yoshimi Nishijima,Naoki Inoue,Hayato Ikota,Akira Iwase,Hideaki Yokoo,Masanao Saio

Cell stem cell 28:1105-1124.e19 PubMed33915078

2021

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Breunig,Jessica Merkle,Martin Wagner,Michael K Melzer,Thomas F E Barth,Thomas Engleitner,Johannes Krumm,Sandra Wiedenmann,Christian M Cohrs,Lukas Perkhofer,Gaurav Jain,Jana Krüger,Patrick C Hermann,Maximilian Schmid,Tamara Madácsy,Árpád Varga,Joscha Griger,Ninel Azoitei,Martin Müller,Oliver Wessely,Pamela G Robey,Sandra Heller,Zahra Dantes,Maximilian Reichert,Cagatay Günes,Christian Bolenz,Florian Kuhn,József Maléth,Stephan Speier,Stefan Liebau,Bence Sipos,Bernhard Kuster,Thomas Seufferlein,Roland Rad,Matthias Meier,Meike Hohwieler,Alexander Kleger

Oncology letters 20:80 PubMed32863913

2020

miR-375-3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor-1β.

Applications

Unspecified application

Species

Unspecified reactive species

Kunpeng Chang,Zhenxing Wei,Hua Cao

제품이 사용된 논문 모두 보기

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Product promise

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