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AB128912

Anti-HNF-1B antibody [EPR6334(2)]

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(7 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal HNF-1B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of human liver tissue staining HNF-1B wth ab128912 with 1/100 dilution (4.6 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of rat liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of mouse liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)
  • WB

Unknown

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

All lanes:

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (ab128912) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

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관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Carrier free

    Anti-HNF-1B antibody [EPR6334(2)] - BSA and Azide free

  • 660 APC

    APC Anti-HNF-1B antibody [EPR6334(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HNF-1B antibody [EPR6334(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HNF-1B antibody [EPR6334(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HNF-1B antibody [EPR6334(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HNF-1B antibody [EPR6334(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-HNF-1B antibody [EPR6334(2)]

  • 578 PE

    PE Anti-HNF-1B antibody [EPR6334(2)]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6334(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB236759

Anti-HNF-1B antibody [CL0374]

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

  • 0
  • 0
Primary Antibodies

AB213149

Anti-HNF-1B antibody [EPR18644-13]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (AB213149)

  • 5
  • 1
Primary Antibodies

AB213150

Anti-HNF-1B antibody [EPR18644-37]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] (AB213150)

  • 0
  • 0
Primary Antibodies

AB238980

Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)

  • 4
  • 1

보완 제품

Assay Kits

AB83374

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

  • 0
  • 0
Primary Antibodies

AB185966

Anti-SOX9 antibody [EPR14335-78]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (AB185966)

  • 5
  • 30
Primary Antibodies

AB181604

Anti-HNF-4-alpha antibody [EPR16885] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] - ChIP Grade (AB181604)

  • 5
  • 4
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

  • 5
  • 43
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.
Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).
See full target information HNF1B

대체 명칭 보기

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

Scientific reports 14:22751 PubMed39349582

2024

Protective effects of cilostazol via the HNF1α/FXR signalling pathway and anti-apoptotic mechanisms in a rat model of estrogen-induced intrahepatic cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Marwa Hassan,Maha B Salem,Olfat A Hammam,Mohamed ElZallat

Cell regeneration (London, England) 13:19 PubMed39347883

2024

Expression levels and stoichiometry of Hnf1β, Emx2, Pax8 and Hnf4 influence direct reprogramming of induced renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xueli Hu,Jianjian Sun,Meng Wan,Bianhong Zhang,Linhui Wang,Tao P Zhong

Physiological genomics 55:565-577 PubMed37720991

2023

Polycystin-2-dependent transcriptome reveals early response of autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Jun Jung,Eryn E Dixon,Richard Coleman,Terry Watnick,Jeremy F Reiter,Patricia Outeda,Valeriu Cebotaru,Owen M Woodward,Paul A Welling

Cell cycle (Georgetown, Tex.) 21:1233-1248 PubMed35230916

2022

LncRNA4474 inhibits renal fibrosis by regulating hepatocyte nuclear factor-1β through miR-615 modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhu,Zhenyu Wang,Zuohui Liang,Shuangyan Xu,Yirong Teng,Xiaolan Li,Yong Zeng

The Journal of biological chemistry 295:17560-17572 PubMed33453998

2021

Hepatocyte nuclear factor 1β suppresses canonical Wnt signaling through transcriptional repression of lymphoid enhancer-binding factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Siu Chiu Chan,Sachin S Hajarnis,Sophia M Vrba,Vishal Patel,Peter Igarashi

Scientific reports 8:12960 PubMed30154523

2018

Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Kevin Van Bortle,Yue Zhang,Ming-Tao Zhao,Joe Z Zhang,Benjamin S Geller,Joshua J Gruber,Chao Jiang,Joseph C Wu,Michael P Snyder
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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