Anti-HNF4A Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF4A Antibody (AB327183)

Immunohistochemical staining of human kidney using ab327183 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in cells in tubules.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF4A Antibody (AB327183)

Immunohistochemical staining of human duodenum using ab327183 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF4A Antibody (AB327183)

Immunohistochemical staining of human pancreas using ab327183 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in exocrine glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF4A Antibody (AB327183)

Immunohistochemistry analysis in human duodenum and tonsil tissues using ab327183 at 1 : 50–1 : 200 dilution. Corresponding HNF4A RNA-seq data are presented for the same tissues.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNF4A Antibody (AB327183)

Immunofluorescent staining of human cell line CACO-2 using ab327183 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• ChIP

Supplier Data

ChIP - Anti-HNF4A Antibody (AB327183)

ChIP-Exo-Seq composite graph for ab327183 tested in HepG2 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-HNF4A Antibody (AB327183)

Western blot analysis in human cell lines Caco-2 and PC-3 using ab327183 at 0.04–0.4 µg/ml. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-HNF4A Antibody (ab327183)

Lane 2:

Human cell lines Caco-2

Lane 3:

Human cell lines PC-3

Predicted band size: 52.78 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF4A Antibody (AB327183)

Immunohistochemical staining of human tonsil using ab327183 at 1 : 50–1 : 200 dilution, showing no positivity as expected.