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AB240965

Anti-HNF6 antibody [EPR14976] - BSA and Azide free

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Rabbit Recombinant Monoclonal HNF6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

HNF6, HNF6A, ONECUT1, Hepatocyte nuclear factor 6, HNF-6, One cut domain family member 1, One cut homeobox 1

1 이미지
Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (AB240965)
  • WB

Supplier Data

Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (AB240965)

All lanes:

Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (ab240965) at 1/1000 dilution

Lane 1:

Human fetal liver cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14976

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

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  • 12
Primary Antibodies

AB231092

Anti-REG1 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG1 alpha antibody (AB231092)

  • 0
  • 0
Primary Antibodies

AB283568

Anti-CGRP-I+CGRP-II antibody [EPR23804-95]

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  • 5
  • 1
Primary Antibodies

AB282459

Anti-Insulin antibody [RM1019]

RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [RM1019] (AB282459)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab240965 is the carrier-free version of ab186743.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hepatocyte nuclear factor 6 (HNF6) also known as Onecut1 is a transcription factor that functions to regulate gene expression. It has a molecular mass of approximately 55 kDa. HNF6 is expressed in the liver pancreas and several other tissues influencing the development and function of these organs. It plays a significant role in regulating genes involved in various cellular processes by binding to DNA sequences and controlling transcription.
Biological function summary

This transcription factor controls the development and maturation of pancreatic islets and hepatocytes. It does not usually function as part of a larger protein complex but it directly influences genes that are vital for these processes. HNF6 ensures proper liver cell differentiation and has an important role in managing glucose metabolism and bile acid homeostasis in tissues where it is expressed.

Pathways

Hepatocyte nuclear factor 6 is integral to the glucose metabolism and bile acid biosynthesis pathways. It interacts with other transcription factors such as HNF4α and FOXA2 forming a regulatory network. HNF6 affects gene expression involved in maintaining metabolic balance and liver detoxification processes illustrating its critical influence on metabolic function within these pathways.

HNF6 links to diabetes and liver diseases. Its modulation of pancreatic development and function implicates it in both type 1 and type 2 diabetes often involving its interaction with other transcription factors like PDX1 and MafA. Similarly changes in HNF6 expression affect liver diseases such as cholestasis where it impacts bile acid regulation and liver cell function.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
See full target information ONECUT1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 21:2209-2219 PubMed32186759

2020

PDGFD induces ibrutinib resistance of diffuse large B‑cell lymphoma through activation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Jin,Leiping Wang,Zhonghua Tao,Jian Zhang,Fangfang Lv,Junning Cao,Xichun Hu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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