AB316365

Anti-hnRNP C1/C2 antibody [4F4]

Recombinant
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Mouse Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPC.

대체 명칭 보기

HNRPC, HNRNPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

Immunohistochemistry analysis using ab316365 on human breast tissue.

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Supplier Data

Western blot - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

(0.01 µg/ml) staining in HeLa (A), HEK293 (B), (0.000 1µg/ml) staining in HepG2 (C) and K562 (D) cells lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Lane 1:

Western blot - Anti-hnRNP C1/C2 Antibody [4F4] (ab316365) at 0.01 µg/mL

Lanes 2 - 4:

Western blot - Anti-hnRNP C1/C2 Antibody [4F4] (ab316365) at 0.0001 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HEK293 cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

K562 cell lysate

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Exposure time: 1hr

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

4F4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HNRNPC.

P07910

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1 month

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C comprises two alternatively spliced proteins C1 protein and C2 protein which have molecular weights of approximately 39 kDa and 41 kDa respectively. These proteins function mainly in the nucleus and are expressed ubiquitously in various tissues. They play a fundamental role in mRNA processing including pre-mRNA splicing polyadenylation and stabilization. As components of the hnRNP complex they bind to nascent RNA transcripts to regulate their maturation and transport.

Biological function summary

HnRNP C1/C2 participates in the assembly of large ribonucleoprotein complexes that are essential for mRNA metabolism. It acts as a core component of the spliceosome machinery where it influences the alternative splicing of pre-mRNA. Apart from its structural role hnRNP C1/C2 may contribute to the proper assembly and functioning of splicing factors and other proteins such as 4f4 within the nucleus suggesting its important role in gene expression regulation.

Pathways

HnRNP C1/C2 is involved in the RNA processing pathway directly impacting mRNA export and stability. By interacting with other proteins such as serine/arginine-rich splicing factors it modulates the splicing accuracy and efficiency of various transcripts. This participation in the spliceosome pathway emphasizes its importance in ensuring efficient gene expression and processing. hnRNP C1/C2 might also interact with various transcription machinery components linking RNA processing to transcriptional regulation.

HnRNP C1/C2 links to neurological diseases and certain cancers. In neurological disorders misregulation of hnRNP C1/C2 can result in aberrant mRNA splicing which may contribute to the pathogenesis of diseases like spinal muscular atrophy. Its involvement in cancer is related to its ability to modulate gene expression and cellular proliferation often observed in tumors with disrupted mRNA processing pathways. HnRNP C1/C2 interactions with proteins such as the tumor suppressor p53 may influence cell cycle regulation and contribute to tumor progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed : 8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed : 12509468, PubMed : 16010978, PubMed : 7567451, PubMed : 8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed : 8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed : 25719671).

See full target information HNRNPC

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP C1/C2 antibody [4F4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

Western blot - Anti-hnRNP C1/C2 Antibody [4F4] (AB316365)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise