AB197023

Anti-HNRNPA0 antibody [EP16085]

RabMAb
Recombinant
KO Validated
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal HNRNPA0 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

대체 명칭 보기

HNRPA0, HNRNPA0, Heterogeneous nuclear ribonucleoprotein A0, hnRNP A0

8 이미지

Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] (AB197023)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1 : ab197023 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Intracellular Flow Cytometry analysis of HeLa cells labelling HNRNPA0 (red) with purified ab197023 at dilution of 1/150. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Immunohistochemical analysis of paraffin-embedded Mouse spleen labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Mouse spleen tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution

All lanes:

293 (Human embryonic kidney) lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,34 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma) lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,34 kDa

false

Lab

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Lanes 1-3 : Merged signal (red and green). Green - ab197023 observed at 32,34 kDa. Red - loading control ab8245 observed at 36 kDa.

ab197023 Anti-HNRNPA0 antibody [EP16085] was shown to specifically react with HNRNPA0 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266314 (knockout cell lysate ab257989) was used. Wild-type and HNRNPA0 knockout samples were subjected to SDS-PAGE. ab197023 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPA0 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPA0 knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-hnrnpa0-knockout-hek-293t-cell-line-ab266314'>ab266314</a>)

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,34 kDa

false

Supplier Data

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cells) lysate at 10 µg

Lane 4:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP16085

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0) is a protein involved in RNA binding and regulation of RNA metabolism. Commonly referred to as HNRNPA0 it has a molecular mass close to 34 kDa. This protein is expressed in a variety of tissues including brain liver and spleen suggesting its widespread functional relevance. HNRNPA0 plays a role in the post-transcriptional regulation of gene expression by influencing mRNA stability and splicing.

Biological function summary

HNRNPA0 modulates RNA processing and is actively involved in mRNA decay and stabilization processes. It forms complexes with other hnRNP proteins and interacts with mRNA substrates to regulate their fate within the cell. This protein contributes to gene expression regulation under stress conditions by altering the stability of specific mRNAs. Such regulation is important for maintaining proper cellular responses to external stress factors.

Pathways

HNRNPA0 is involved in the p38 MAPK signaling pathway where it influences the stability of mRNAs encoding inflammatory mediators. It also plays a part in the apoptotic pathway where it interacts with proteins such as TRAF2 and apoptosis as death domain-like protein (ADRP). Through these pathways HNRNPA0 contributes to cellular stress responses inflammation and programmed cell death linking it to essential cellular processes.

HNRNPA0 has been implicated in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) where protein aggregates may form due to dysregulation of HNRNPA0 activity. It is also associated with certain types of cancer such as leukemia where altered expression levels may impact mRNA stability and cell proliferation. Through these disease contexts HNRNPA0 interacts with proteins like TDP-43 in ALS and has connections to proto-oncogenes in cancer highlighting its potential role as a therapeutic target.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post-transcriptional regulation of cytokines mRNAs.

See full target information HNRNPA0

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of virology 98:e0053424 PubMed38899932

2024

The interferon-regulated host factor hnRNPA0 modulates HIV-1 production by interference with LTR activity, mRNA trafficking, and programmed ribosomal frameshifting.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Roesmann,Helene Sertznig,Katleen Klaassen,Alexander Wilhelm,Delia Heininger,Stefanie Heß,Carina Elsner,Rolf Marschalek,Mario L Santiago,Stefan Esser,Kathrin Sutter,Ulf Dittmer,Marek Widera

The EMBO journal 41:e110466 PubMed35307861

2022

Ketogenic HMG-CoA lyase and its product β-hydroxybutyrate promote pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Victoire Gouirand,Tristan Gicquel,Evan C Lien,Emilie Jaune-Pons,Quentin Da Costa,Pascal Finetti,Elodie Metay,Camille Duluc,Jared R Mayers,Stephane Audebert,Luc Camoin,Laurence Borge,Marion Rubis,Julie Leca,Jeremy Nigri,François Bertucci,Nelson Dusetti,Juan L Iovanna,Richard Tomasini,Ghislain Bidaut,Fabienne Guillaumond,Matthew G Vander Heiden,Sophie Vasseur

Translational lung cancer research 8:760-774 PubMed32010555

2020

Silence of S1 RNA binding domain 1 represses cell growth and promotes apoptosis in human non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Guowei Cheng,Lei Deng,Yin Yang,Li Sun,Ping Chen,Xiangling He,Dan Su,Nan Bi,Bin Qiu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNRNPA0 antibody [EP16085]

Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Flow Cytometry (Intracellular) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

Western blot - Anti-HNRNPA0 antibody [EP16085] (AB197023)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise