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AB249462

Anti-HOXC13 antibody [EPR5626] - BSA and Azide free

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Rabbit Recombinant Monoclonal HOXC13 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

HOX3G, HOXC13, Homeobox protein Hox-C13, Homeobox protein Hox-3G

2 이미지
Immunoprecipitation - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (AB249462)
  • IP

Unknown

Immunoprecipitation - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (AB249462)

This data was developed using ab168368, the same antibody clone in a different buffer formulation.Detection of HOXC13 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab168368 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (ab249462)

Predicted band size: 35 kDa

false

Western blot - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (AB249462)
  • WB

Unknown

Western blot - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (AB249462)

This data was developed using ab168368, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HOXC13 antibody [EPR5626] - BSA and Azide free (ab249462) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 35 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5626

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Assay Kits

AB228554

Cell Counting Kit 8 (WST-8 / CCK8)

Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (AB228554)

  • 5
  • 4
Primary Antibodies

AB188470

Anti-Dnmt3a antibody [EPR18455]

20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] (AB188470)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab249462 is the carrier-free version of ab168368.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HOXC13 also known as Homeobox protein Hox-C13 is a transcription factor belonging to the homeobox gene family. This protein plays a significant role in regulating gene expression influencing cellular proliferation and differentiation. HOXC13 has a mass of approximately 37 kDa and is expressed in specific tissues including hair follicles the skin and certain regions of the central nervous system. The protein contains a homeodomain which allows it to bind DNA and control the transcription of genes essential for proper development.
Biological function summary

HOXC13 influences the development and maintenance of hair and skin by regulating the expression of keratin genes. As a member of the homeobox gene complex it contributes to the establishment of body plans during embryonic development. HOXC13 acts not alone but as part of transcriptional complexes that modify expression patterns of target genes ensuring proper structural attributes of tissues. Its activity impacts cellular differentiation impacting phenotypic characteristics such as hair texture and skin structure.

Pathways

HOXC13 plays a role in the Wnt signaling pathway and epidermal differentiation processes. It interacts with other HOX proteins such as HOXC8 and HOXD13 contributing to the regulation of gene networks involved in skin and appendage formation. These pathways dictate cellular fate ensuring coordinated development and function of keratinocytes in the epidermis as well as hair follicle morphogenesis and cycling.

Alterations in HOXC13 expression or function have been associated with ectodermal dysplasia and hair abnormalities. Mutations or dysregulation of this protein disrupt normal hair and skin development often leading to phenotypes of clinical importance like hypotrichosis. HOXC13 has been implicated in ectodermal dysplasias through its regulatory relationships with genes responsible for keratin synthesis highlighting its importance in integumentary system anomalies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor which plays a role in hair follicle differentiation. Regulates FOXQ1 expression and that of other hair-specific genes (By similarity).
See full target information HOXC13
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
제품이 사용된 논문 모두 보기
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Product promise

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