Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with ab322271 at 1/2000 (0.49ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322271 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution. -ve control 2 : ab9106 Anti-Myc Rabbit polyclonal antibody at 1/200 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271)

Indirect ELISA analysis of ab322271 at 2 ug/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : HPV 59 L1 protein, HPV 18 L1 protein, HPV 16 L1 protein, HPV 6B L1 protein, HPV 11 L1 protein.

Antigen concentration : 1000-0 ug/ml