AB100969

Anti-HPV18 E2 antibody [2E7]

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(2 제품이 사용된 논문 )

Mouse Monoclonal VE2 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human papillomavirus samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E2.

대체 명칭 보기

Regulatory protein E2, E2, E2, Regulatory protein E2, Human papillomavirus type 18 E2 protein

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (AB100969)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (AB100969)

Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

Unknown

Western blot - Anti-HPV18 E2 antibody [2E7] (AB100969)

All lanes:

Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

Lane 1:

lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein

Lane 2:

lysates of plain U20S cells

Lane 3:

lysates of plain HeLa cells

Lane 4:

lysates of U20S transfected with plasmid expressing HPV11 E2 protein

Lane 5:

marker

Predicted band size: 41 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2E7

Isotype

IgG

Carrier free

Reacts with

Human papillomavirus

Applications

WB, ELISA, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E2.

P06790

Epitope

Mapped to amino acids 1-83.

Specificity

Reacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "0.1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from bacteria by affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

See full target information E2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Archives of virology 164:1815-1827 PubMed30631960

2019

Intratype variants of the E2 protein from human papillomavirus type 18 induce different gene expression profiles associated with apoptosis and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Alma Mariana Fuentes-González,J Omar Muñoz-Bello,Joaquín Manzo-Merino,Adriana Contreras-Paredes,Abraham Pedroza-Torres,Jorge Fernández-Retana,Carlos Pérez-Plasencia,Marcela Lizano

International journal of biological sciences 14:21-35 PubMed29483822

2018

Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage.

Applications

Species

Unspecified reactive species

Alfredo Cruz-Gregorio,Joaquín Manzo-Merino,María Cecilia Gonzaléz-García,José Pedraza-Chaverri,Omar Noel Medina-Campos,Mahara Valverde,Emilio Rojas,María Alexandra Rodríguez-Sastre,Claudia María García-Cuellar,Marcela Lizano

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HPV18 E2 antibody [2E7]

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (AB100969)

Western blot - Anti-HPV18 E2 antibody [2E7] (AB100969)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise