AB3553

HRP Anti-6X His tag® antibody

Name: HRP Anti-6X His tag® antibody
Brand: Abcam Limited
SKU: AB3553
Rating: 5 (1 reviews)

(1 리뷰)

(13 제품이 사용된 논문 )

Anti-6X His tag® antibody conjugated to HRP is a chicken polyclonal antibody suitable for ELISA, Western blot.

1 이미지

ELISA - HRP Anti-6X His tag® antibody (AB3553)

ELISA

Supplier Data

ELISA - HRP Anti-6X His tag® antibody (AB3553)

Antigen concentration : 1000 ng/well. ab3553 was incubated 60 minutes. Developed with TMB. Microplate reader set at 450nm.

주요 정보

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Light chain 타입

unknown

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

WB, ELISA

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1000.</p>" } } }

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제품 세부 정보

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information 6X His tag®

대체 명칭 보기

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

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제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:496 PubMed40617804

2025

Nedd4-2 ablation in kidney improves glycaemic control in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jantina A Manning,Shilpanjali Jesudason,Paul A B Moretti,Stuart M Pitson,Angela S Y Chou,Meriam Shabbar,Sonia Saad,Carol Pollock,Sharad Kumar

Cells 13: PubMed38534369

2024

Potential Utility of Urinary Follistatin as a Non-Invasive Indicator of Acute Tubular Damage in Patients with Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Izumi Nagayama,Kaori Takayanagi,Daisuke Nagata,Hajime Hasegawa,Akito Maeshima

Cells 12: PubMed36899937

2023

Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Izumi Nagayama,Kaori Takayanagi,Hajime Hasegawa,Akito Maeshima

Cell reports 41:111673 PubMed36417869

2022

A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γ.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Tang,Gunjan Arora,Jaqueline Matias,Thomas Hart,Yingjun Cui,Erol Fikrig

eLife 10: PubMed34783654

2021

The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Tang,Yongguo Cao,Gunjan Arora,Jesse Hwang,Andaleeb Sajid,Courtney L Brown,Sameet Mehta,Alejandro Marín-López,Yu-Min Chuang,Ming-Jie Wu,Hongwei Ma,Utpal Pal,Sukanya Narasimhan,Erol Fikrig

International journal of molecular sciences 22: PubMed33498219

2021

MST3 Involvement in Na and K Homeostasis with Increasing Dietary Potassium Intake.

Applications

Unspecified application

Species

Unspecified reactive species

Chee-Hong Chan,Sheng-Nan Wu,Bo-Ying Bao,Houng-Wei Li,Te-Ling Lu

Cell death and differentiation 27:1832-1843 PubMed31802037

2019

Dietary sodium modulates nephropathy in Nedd4-2-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jantina A Manning,Sonia S Shah,Tanya L Henshall,Andrej Nikolic,John Finnie,Sharad Kumar

Aging cell 18:e13004 PubMed31318148

2019

Wnt/β-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Miao,Jiafeng Liu,Jing Niu,Yunfang Zhang,Weiwei Shen,Congwei Luo,Yahong Liu,Chuanjiang Li,Hongyan Li,Peiliang Yang,Youhua Liu,Fan Fan Hou,Lili Zhou

iScience 11:375-387 PubMed30660105

2019

In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Burnside,Andrew Schoenrock,Houman Moteshareie,Mohsen Hooshyar,Prabh Basra,Maryam Hajikarimlou,Kevin Dick,Brad Barnes,Tom Kazmirchuk,Matthew Jessulat,Sylvain Pitre,Bahram Samanfar,Mohan Babu,James R Green,Alex Wong,Frank Dehne,Kyle K Biggar,Ashkan Golshani

Cell host & microbe 23:523-535.e5 PubMed29649443

2018

Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Srdjan M Dragovic,Tolulope A Agunbiade,Marianna Freudzon,Jing Yang,Andrew K Hastings,Tyler R Schleicher,Xia Zhou,Sam Craft,Yu-Min Chuang,Floricel Gonzalez,Youquan Li,Gabriela Hrebikova,Abhai Tripathi,Godfree Mlambo,Lionel Almeras,Alexander Ploss,George Dimopoulos,Erol Fikrig

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody

ELISA - HRP Anti-6X His tag® antibody (AB3553)

주요 정보

Host species

Clonality

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (13)

Product promise