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AB325038

HRP Anti-Aquaporin 2 antibody [EPR21080]

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Rabbit Recombinant Monoclonal Aquaporin 2 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Aquaporin 2 with ab325038 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle.
The section was incubated with ab325038 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Aquaporin 2 with ab325038 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on luminal surface of collecting duct in human kidney.
The section was incubated with ab325038 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Aquaporin 2 with ab325038 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on luminal surface of collecting duct in rat kidney.
The section was incubated with ab325038 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Aquaporin 2 antibody [EPR21080] (AB325038)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 2 with ab325038 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on luminal surface of collecting duct in mouse kidney.
The section was incubated with ab325038 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21080

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Mouse, Rat, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

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Primary Antibodies

AB225153

Alexa Fluor® 647 Anti-CD103 antibody [EPR4166(2)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD103 antibody [EPR4166(2)] (AB225153)

  • 4
  • 2
Primary Antibodies

AB312904

Alexa Fluor® 647 Anti-NKG2D antibody [EPR24072-342]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NKG2D antibody [EPR24072-342] (AB312904)

  • 0
  • 0
Primary Antibodies

AB300745

Alexa Fluor® 647 Anti-NKG2A antibody [EPR23737-127]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NKG2A antibody [EPR23737-127] (AB300745)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.
Biological function summary

Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.

Pathways

Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.

Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed : 15509592, PubMed : 7510718, PubMed : 7524315, PubMed : 8140421, PubMed : 8584435). Plays an essential role in renal water homeostasis (PubMed : 15509592, PubMed : 7524315, PubMed : 8140421). Could also be permeable to glycerol (PubMed : 8584435).
See full target information AQP2

대체 명칭 보기

Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD, AQP2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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