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AB197905

HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker

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Anti-ATPB antibody [3D5] conjugated to HRP - suitable for Western blot, and reacts with human samples. Clone [3D5] is a mouse monoclonal antibody cited in 11 publications.
1 이미지
Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB197905)
  • WB

Lab

Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB197905)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905) at 1/5000 dilution

Lane 1:

Human heart tissue lysate - mitochondrial extract (<a href='/ko/products/unavailable/human-heart-tissue-lysate-mitochondrial-extract-ab110337'>ab110337</a>) at 5 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/ko/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 56 kDa

Observed band size: 52 kDa

true

Exposure time: 2s

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Unconjugated

    Anti-ATPB antibody [3D5] - Mitochondrial Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ATPB antibody [3D5] - Mitochondrial Marker

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3D5

Isotype

IgG1

Light chain 타입

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

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대체품

Primary Antibodies

AB170948

Anti-ATPB antibody [EPR11991] - Mitochondrial Marker

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ATPB antibody [EPR11991] - Mitochondrial Marker (AB170948)

  • 0
  • 1
Primary Antibodies

AB223436

Alexa Fluor® 647 Anti-ATPB antibody [EPR11990] - Mitochondrial Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB223436)

  • 0
  • 0

보완 제품

Assay Kits

AB109714

ATP synthase Activity Assay Kit (Colorimetric)

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

  • 5
  • 1
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
Primary Antibodies

AB209606

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)

  • 5
  • 2
Primary Antibodies

AB203832

Anti-UQCRC2 antibody [EPR13051]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (AB203832)

  • 5
  • 1
Primary Antibodies

AB14744

Anti-COX IV antibody [20E8C12] - Mitochondrial Marker

Lab Essentials

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

  • 5
  • 23
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Caenorhabditis elegans": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
보관 버퍼
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.
Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable) (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the subunit alpha (ATP5F1A), forms the catalytic core in the F(1) domain (PubMed : 37244256).
See full target information ATP5F1B

대체 명칭 보기

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 10:7795 PubMed32385361

2020

Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Miao Yu,Xuanze Shang,My Hong Hoai Nguyen,Shanmuganathan Balakrishnan,Rachel Sager,Huaiyu Hu
제품이 사용된 논문 모두 보기
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Product promise

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