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AB20272

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

HRP Anti-beta Actin 항체 [mAbcam 8226] - Loading Control

5

(18 리뷰들)

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(377 제품이 사용된 논문 )

Anti-beta Actin antibody [mAbcam 8226] conjugated to HRP - suitable for Western blot, and reacts with mouse, human, rat samples. Clone [mAbcam 8226] is a mouse monoclonal antibody cited in 13 publications.
3 이미지
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)
  • WB

Unknown

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

human testis lysate at 20 µg

Lane 2:

human brain lysate at 20 µg

Lane 3:

human tonsil lysate at 20 µg

Lane 4:

mouse brain lysate at 20 µg

Lane 5:

mouse spleen lysate at 20 µg

Predicted band size: 41 kDa

true

Exposure time: 2min

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)
  • WB

Project

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lanes 2 and 6:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lanes 3 and 7:

Western blot - NIH/3T3 whole cell lysate (<a href='/ko/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lanes 4 and 8:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 1min

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)
  • WB

Project

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

3T3 whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 30s

관련 conjugated 항체와 다양한 조성의 항체 (4)

  • Unconjugated

    Anti-beta Actin antibody [mAbcam 8226] - Loading Control

  • Carrier free

    Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free

  • 702 Alexa Fluor® 680

    Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

  • 805 Alexa Fluor® 790

    Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 8226

Isotype

IgG1

Light chain 타입

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.</p>" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

제품 세부 정보

What is this antibody validated in?
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of beta Actin?
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) is often used as a loading control in Western blot. It specifically detects a band for beta Actin (UniProt: P60709) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) was first used in a scientific publication in 2005 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) has over 15 independent reviews from customers.

Related products
Antibody clone mAbcam 8226 is also available pre-conjugated to a variety of labels for your convenience – Anti-beta Actin HRP [mAbcam 8226] - Loading Control (ab20272).

Other related products
We have a range of other formats of antibody clone [mAbcam 8226] also available for your convenience: ab8226, HRP - ab20272, Alexa Fluor® 680 - ab184092, Alexa Fluor® 790 - ab184576, Carrier free - ab264083

특성 및 보관 정보

제형
Liquid
Purity
IgG fraction
보관 버퍼
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB

대체 명칭 보기

Beta-actin, ACTB

제품이 사용된 논문 (377)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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Science advances 11:eadu4270 PubMed40749060

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International journal of molecular sciences 26: PubMed40724911

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Biochemical Insights into the Effects of a Small Molecule Drug Candidate on Imatinib-Induced Cardiac Inflammation.

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Renáta Szabó,Denise Börzsei,András Nagy,Viktória Kiss,Zoltán Virág,Gyöngyi Kis,Nikoletta Almási,Szilvia Török,Médea Veszelka,Mária Bagyánszki,Nikolett Bódi,Bence Pál Barta,Patrícia Neuperger,Gabor J Szebeni,Csaba Varga

Journal of thoracic disease 17:4249-4263 PubMed40688308

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regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an model of atrial fibrillation.

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Species

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Yimeng Zhou,Lianzhi Zhang,Wenjia Zhang,Qunlin Gong,Zhong Zhang,Nan Xu,Ashley R Wilson-Smith,Nannan Chen

Nature neuroscience 28:1688-1703 PubMed40659845

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Species

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Alberto Ardura-Fabregat,Lance Fredrick Pahutan Bosch,Emile Wogram,Omar Mossad,Roman Sankowski,Philipp Aktories,Lina Kieger,James Cook,Dilara Hasavci,Hatice Ulupinar,Daniel Brock,Fang Wang,Nicola Iovino,Samuel Wald,Sebastian Preissl,Bahtiyar Yilmaz,Daniel Schnepf,Andrew J Macpherson,Thomas Blank,Katrin Kierdorf,Marco Prinz

International journal of molecular sciences 26: PubMed40649891

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Targeting PAD4: A Promising Strategy to Combat β-Cell Loss in Type 1 Diabetes.

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Species

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Hsu Lin Kang,András Szász,Zsuzsanna Valkusz,Tamás Várkonyi,Anikó Pósa,Krisztina Kupai

Brain communications 7:fcaf224 PubMed40574971

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Glycine-to-aspartic acid mutation at codon 51 in disrupts the synaptic localisation of α-synuclein and enhances its propensity for synucleinopathy.

Applications

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Species

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Stephen West,Ammar Natalwala,Karamjit Singh Dolt,Douglas J Lamont,Melanie McMillan,Kelvin Luk,Tomoji Mashimo,Tilo Kunath

Nature communications 16:5209 PubMed40473625

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Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca import and H efflux.

Applications

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Species

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Ran Chen,Bin Liu,Dawid Jaślan,Lucija Kucej,Veronika Kudrina,Belinda Warnke,Yvonne E Klingl,Arnas Petrauskas,Sandra Prat Castro,Kenji Maeda,Christian Grimm,Marja Jäättelä

Cell reports. Medicine 6:102135 PubMed40398389

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MPXV infection activates cGAS-STING signaling and IFN-I treatment reduces pathogenicity of mpox in CAST/EiJ mice and rhesus macaques.

Applications

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Species

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Lin Zhu,Qi Liu,Yongzhi Hou,Baoying Huang,Dong Zhang,Zhe Cong,Jianrong Ma,Na Li,Jiahan Lu,Jingjing Zhang,Lingyan Zhang,Ting Chen,Qiang Wei,Jiangning Liu,Wenjie Tan,Jing Xue
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