AB81958

HRP Anti-CHREBP antibody

Name: HRP Anti-CHREBP antibody
Brand: Abcam Limited
SKU: AB81958
Rating: 4 (1 reviews)

(1 리뷰)

(7 제품이 사용된 논문 )

Anti-CHREBP antibody conjugated to HRP is a rabbit polyclonal antibody suitable for ICC, ICC/IF, IHC-P, Western blot, and reacts with human samples.

3 이미지

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).

Unknown

Western blot - HRP Anti-CHREBP antibody (AB81958)

5-10 second film exposure.

All lanes:

anti CHREBP (unconjugated form of ab81958)

All lanes:

human hepatocyte lysate at 20 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

Unknown

Western blot - HRP Anti-CHREBP antibody (AB81958)

7% SDS gel.

All lanes:

anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

Lane 1:

liver nuclear extracts from well fed rats at 10 µg

Lane 2:

liver nuclear extracts from well fed rats at 5 µg

Lane 3:

liver nuclear extracts from well fed rats at 1 µg

Lane 4:

liver nuclear extracts from well fed rats at 0.5 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Synthetic Peptide within Human MLXIPL aa 800-900. The exact immunogen used to generate this antibody is proprietary information.

Q9NP71

Specificity

A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified rabbit antisera.

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Glucose-responsive transcription activator that regulates fatty acid synthesis and glycolysis. Key determinant of systemic insulin sensitivity and glucose homeostasis. Important for the expression of fatty acid synthetic enzymes, including PC/Pcx, APOC4/Acl, ACACA/Acc1 and FASN/Fas (By similarity). Important for glucose-induced expression of L-type pyruvate kinase/PKLR (By similarity). Binds to the canonical and non-canonical E box DNA sequences 5'-CACGTG-3' and 5'-CACGCG-3' (By similarity). May also act as a transcriptional repressor (By similarity).

See full target information MLXIPL

대체 명칭 보기

BHLHD14, MIO, WBSCR14, MLXIPL, Carbohydrate-responsive element-binding protein, ChREBP, Class D basic helix-loop-helix protein 14, MLX interactor, MLX-interacting protein-like, WS basic-helix-loop-helix leucine zipper protein, Williams-Beuren syndrome chromosomal region 14 protein, bHLHd14, WS-bHLH

style

left-column

제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aquaculture nutrition 2023:6672985 PubMed37520290

2023

Sequential Activations of ChREBP and SREBP1 Signals Regulate the High-Carbohydrate Diet-Induced Hepatic Lipid Deposition in Gibel Carp ().

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Gong,Longwei Xi,Yulong Liu,Qisheng Lu,Zhimin Zhang,Haokun Liu,Junyan Jin,Yunxia Yang,Xiaoming Zhu,Shouqi Xie,Dong Han

The Journal of nutrition 150:1790-1798 PubMed32470978

2020

Dietary Glucose Increases Glucose Absorption and Lipid Deposition via SGLT1/2 Signaling and Acetylated ChREBP in the Intestine and Isolated Intestinal Epithelial Cells of Yellow Catfish.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhao,Shui-Bo Yang,Guang-Hui Chen,Yi-Huan Xu,Yi-Chuang Xu,Zhi Luo

Journal of biochemistry 167:371-377 PubMed31782782

2019

TXNIP induced by MondoA, rather than ChREBP, suppresses cervical cancer cell proliferation, migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Junhua Zhang,Xingbo Tian,Huifang Yin,Songshu Xiao,Shuijing Yi,Youzhong Zhang,Fei Zeng

Cell reports 24:1597-1609 PubMed30089269

2018

Elimination of Age-Associated Hepatic Steatosis and Correction of Aging Phenotype by Inhibition of cdk4-C/EBPα-p300 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong Nguyen,Leila Valanejad,Ashley Cast,Mary Wright,Jose M Garcia,Hashem B El-Serag,Rebekah Karns,Nikolai A Timchenko

Open biology 6: PubMed27249798

2016

The role of the glucose-sensing transcription factor carbohydrate-responsive element-binding protein pathway in termite queen fertility.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

David Sillam-Dussès,Robert Hanus,Michael Poulsen,Virginie Roy,Maryline Favier,Mireille Vasseur-Cognet

Journal of clinical biochemistry and nutrition 57:244-53 PubMed26566312

2015

Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice.

Applications

Species

Unspecified reactive species

Tetsuya Sujishi,Shinya Fukunishi,Masaaki Ii,Ken Nakamura,Keisuke Yokohama,Hideko Ohama,Yusuke Tsuchimoto,Akira Asai,Yasuhiro Tsuda,Kazuhide Higuchi

PPAR research 2013:865604 PubMed23533380

2013

Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Huang,Avery L McIntosh,Gregory G Martin,Anca D Petrescu,Kerstin K Landrock,Danilo Landrock,Ann B Kier,Friedhelm Schroeder

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CHREBP antibody

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

Western blot - HRP Anti-CHREBP antibody (AB81958)

Western blot - HRP Anti-CHREBP antibody (AB81958)

주요 정보

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise