JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB194313

HRP Anti-CREB antibody [E306]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal CREB antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CREB antibody [E306] (AB194313)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CREB antibody [E306] (AB194313)

IHC image of CREB staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194313 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

E306

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB47373

Anti-CREB (phospho S129) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB (phospho S129) antibody (AB47373)

  • 0
  • 0
Primary Antibodies

AB178322

Anti-CREB antibody [LB9]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [LB9] (AB178322)

  • 4
  • 1
Primary Antibodies

AB32096

Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] (AB32096)

  • 4
  • 14
Primary Antibodies

AB220798

Anti-CREB (phospho S133) antibody [E113] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] - BSA and Azide free (AB220798)

  • 0
  • 0
Primary Antibodies

AB194687

Anti-CREB (phospho S133) antibody

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody (AB194687)

  • 0
  • 0
Primary Antibodies

AB219741

HRP Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CREB (phospho S133) antibody [E113] (AB219741)

  • 0
  • 0

보완 제품

Primary Antibodies

AB219741

HRP Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CREB (phospho S133) antibody [E113] (AB219741)

  • 0
  • 0
Primary Antibodies

AB181591

Anti-Cyclin A2 antibody [EPR17351]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

  • 5
  • 3
Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

  • 5
  • 3
Primary Antibodies

AB233078

Anti-PPAR alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody (AB233078)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
See full target information CREB1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Epigenetics & chromatin 18:2 PubMed39800758

2025

H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.

Applications

Unspecified application

Species

Unspecified reactive species

Wilhelm Bouchereau,Hong-Thu Pham,Worawalan Samruan,Van-Hong Vu,Thierry Joly,Marielle Afanassieff,Pierre Savatier,Rangsun Parnpai,Nathalie Beaujean
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com