AB2493

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Name: HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
Brand: Abcam Limited
SKU: AB2493
Rating: 5 (1 reviews)

(1 리뷰)

(4 제품이 사용된 논문 )

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody conjugated to HRP is a rabbit polyclonal antibody suitable for ELISA, Western blot.

1 이미지

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

ELISA

Supplier Data

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

Antigen concentration : 1000 ng/well. ab2493 was incubated for 60 minutes. Developed with TMB. Microplate reader set at 450nm.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

WB, ELISA

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information DDDDK tag (Binds to FLAG® tag sequence)

대체 명칭 보기

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature chemical biology 19:378-388 PubMed36782012

2023

TMEM164 is an acyltransferase that forms ferroptotic C20:4 ether phospholipids.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Reed,Timothy Ware,Haoxin Li,J Fernando Bazan,Benjamin F Cravatt

Infection and immunity 88: PubMed32817331

2020

Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group A .

Applications

Unspecified application

Species

Unspecified reactive species

Paul E Bernard,Amey Duarte,Mikhail Bogdanov,James M Musser,Randall J Olsen

Nucleic acids research 46:6401-6415 PubMed29961805

2018

Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan A Belashov,David W Crawford,Chapin E Cavender,Peng Dai,Patrick C Beardslee,David H Mathews,Bradley L Pentelute,Brian R McNaughton,Joseph E Wedekind

Nucleic acids research 46:7250-7260 PubMed29945249

2018

Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Kling,Thomas Spaller,Jana Schiefner,Doreen Bönisch,Thomas Winckler

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

주요 정보

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise