AB203479

HRP Anti-EAAT3 antibody [EPR6774(B)]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal EAAT3 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

EAAC1, EAAT3, HEAAC1, SLC1A1, Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

1 이미지

Lab

Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (AB203479)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203479 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479) at 1/5000 dilution

Lane 1:

Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 2:

Testis (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 3:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg

Predicted band size: 57 kDa

Observed band size: 70 kDa

true

Exposure time: 8s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6774(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Stable for 12 months at -20°C|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EAAT3 also known as Excitatory Amino Acid Transporter 3 or SLC1A1 is a protein that transports amino acids particularly glutamate across cellular membranes. EAAT3 has a mass of approximately 57 kDa. It is expressed in various tissues including the brain and kidneys. In neurons it accumulates glutamate into the cells which is important for maintaining synaptic transmission balance and preventing excitotoxic damage.

Biological function summary

EAAT3 functions to regulate extracellular levels of glutamate an essential neurotransmitter. The transporter prevents excessive accumulation of glutamate in the synaptic cleft promoting synaptic efficiency and neuron communication. EAAT3 operates as part of a larger family of transporters including EAAT1 EAAT2 and others that collectively maintain glutamate homeostasis in the central nervous system.

Pathways

EAAT3 engages in neurotransmitter metabolism and the glutamatergic synapse. It collaborates with EAAT2 in similar pathways. Together they ensure that excess glutamate is absorbed and reused or degraded safeguarding against excitotoxicity. These pathways are critical for maintaining neuronal health and function.

EAAT3 has links to obsessive-compulsive disorder (OCD) and epilepsy. EAAT3 dysfunction or altered expression can lead to imbalance in neurotransmitter levels contributing to these conditions. Changes in EAAT2 a related transporter also have implications in these disorders. Understanding these interactions helps in exploring EAAT3 as a possible therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 21123949, PubMed : 26690923, PubMed : 33658209, PubMed : 7521911, PubMed : 7914198, PubMed : 8857541). Can also transport L-cysteine (PubMed : 21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 26690923, PubMed : 33658209, PubMed : 7521911, PubMed : 8857541). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 26690923, PubMed : 8857541). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed : 21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity).

See full target information SLC1A1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 16:3525-3530 PubMed30127957

2018

miR-486-5p inhibits cell proliferation and invasion through repressing GAB2 in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sunfeng Yu,Shuang Geng,Yi Hu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-EAAT3 antibody [EPR6774(B)]

Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (AB203479)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise