AB195851

HRP Anti-HDAC2 antibody [Y461]

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal HDAC2 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

2 이미지

Lab

Western blot - HRP Anti-HDAC2 antibody [Y461] (AB195851)

ab195851 was shown to specifically react with HDAC2 in wild-type HAP1 cells as signal was lost in HDAC2 knockout cells. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. ab195851 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-HDAC2 antibody [Y461] (ab195851) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

HDAC2 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Exposure time: 30s

Lab

Western blot - HRP Anti-HDAC2 antibody [Y461] (AB195851)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195851 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

All lanes:

Western blot - HRP Anti-HDAC2 antibody [Y461] (ab195851) at 1/10000 dilution

All lanes:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Predicted band size: 55 kDa

Observed band size: 60 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y461

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HDAC2 or Histone Deacetylase 2 is an important enzyme that plays a role in chromatin remodeling and gene expression regulation. It removes acetyl groups from histone tails allowing chromatin to condense and silencing gene expression. HDAC2 has a molecular weight of approximately 55 kDa. It is expressed in various tissues including brain heart and skeletal muscles indicating its importance in different physiological areas. HDAC2 proteins are often studied using methods like HDAC2 ELISA and HDAC2 anticuero in research settings.

Biological function summary

HDAC2 is involved in cell cycle regulation and differentiation. It is part of the co-repressor complex and interacts with other proteins such as Sin3 and NuRD to repress transcription. These interactions are vital for maintaining normal cellular functions and ensuring proper response to external signals. This protein is also connected to HEK293T cells which are a common model in scientific studies due to their robust expression of proteins like HDAC2.

Pathways

HDAC2 participates in the regulation of transcriptional activity and cellular stress response. It is an important player in the histone modification pathway interacting with proteins such as HDAC1 and transcriptional regulators like REST. HDAC2 is also linked with the Notch signaling pathway which is essential for cell fate decisions. These pathways highlight its role in both maintaining cellular homeostasis and adapting to changes in the cellular environment.

HDAC2 has a significant connection to neurodegenerative diseases and various cancers. Alzheimer's disease for instance shows altered expression levels of HDAC2 affecting synaptic plasticity and memory formation. In cancer HDAC2 interacts with oncogenic proteins such as p53 influencing tumor progression and metastasis. Monitoring HDAC2 levels and activity could provide insights into disease mechanisms and potential therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed : 12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 37137925). Also deacetylates non-histone targets : deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed : 19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed : 21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 35044827).

See full target information HDAC2

대체 명칭 보기

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록and refine your search

The Journal of experimental medicine 221: PubMed38557723

2024

Helper T cell immunity in humans with inherited CD4 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Guérin,Marcela Moncada-Vélez,Katherine Jackson,Masato Ogishi,Jérémie Rosain,Mathieu Mancini,David Langlais,Andrea Nunez,Samantha Webster,Jesse Goyette,Taushif Khan,Nico Marr,Danielle T Avery,Geetha Rao,Tim Waterboer,Birgitta Michels,Esmeralda Neves,Cátia Iracema Morais,Jonathan London,Stéphanie Mestrallet,Pierre Quartier Dit Maire,Bénédicte Neven,Franck Rapaport,Yoann Seeleuthner,Atar Lev,Amos J Simon,Jorge Montoya,Ortal Barel,Julio Gómez-Rodríguez,Julio C Orrego,Anne-Sophie L'Honneur,Camille Soudée,Jessica Rojas,Alejandra C Velez,Irini Sereti,Benjamin Terrier,Nancy Marin,Luis F García,Laurent Abel,Stéphanie Boisson-Dupuis,Joel Reis,Antonio Marinho,Andrea Lisco,Emilia Faria,Christopher C Goodnow,Julia Vasconcelos,Vivien Béziat,Cindy S Ma,Raz Somech,Jean-Laurent Casanova,Jacinta Bustamante,Jose Luis Franco,Stuart G Tangye

Molecules (Basel, Switzerland) 26: PubMed33921211

2021

Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.

Applications

Unspecified application

Species

Unspecified reactive species

Thai Pham,Renjie Liao,Joshua Labaer,Jia Guo

Frontiers in cell and developmental biology 8:614624 PubMed33585449

2021

Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Liao,Manas Mondal,Christopher D Nazaroff,Diego Mastroeni,Paul D Coleman,Joshua Labaer,Jia Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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HRP Anti-HDAC2 antibody [Y461]

Western blot - HRP Anti-HDAC2 antibody [Y461] (AB195851)

Western blot - HRP Anti-HDAC2 antibody [Y461] (AB195851)

관련 conjugated 항체와 다양한 조성의 항체 (9)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise