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AB203464

HRP Anti-TXNRD1 antibody [EPNCIR129]

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Rabbit Recombinant Monoclonal TXNRD1 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

GRIM12, KDRF, TXNRD1, TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TXNRD1 antibody [EPNCIR129] (AB203464)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TXNRD1 antibody [EPNCIR129] (AB203464)

IHC image of TXNRD1 staining in a section of formalin-fixed paraffin-embedded human liver hepatocellular carcinoma tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203464, 1/125 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Western blot - HRP Anti-TXNRD1 antibody [EPNCIR129] (AB203464)
  • WB

Lab

Western blot - HRP Anti-TXNRD1 antibody [EPNCIR129] (AB203464)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203464 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464) at 1/5000 dilution

Lane 1:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

Lane 4:

Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 5:

Liver (Rat) Tissue Lysate at 10 µg

Predicted band size: 71 kDa

Observed band size: 55 kDa

true

Exposure time: 1min

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR129

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB151716

Anti-TXNRD1 antibody [EPR10204(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10204(B)] (AB151716)

  • 0
  • 0

보완 제품

Assay Kits

AB83463

Thioredoxin Reductase Assay Kit (Colorimetric)

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

  • 0
  • 0
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB209606

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)

  • 5
  • 2
Proteins & Peptides

AB204206

Recombinant Human TXNRD1 protein

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/125", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 1 (TXNRD1) also known as TRXR1 is a selenoprotein that plays an important role in maintaining cellular redox balance by catalyzing the reduction of thioredoxin (TRX). It possesses a molecular mass of approximately 55 kDa. TXNRD1 can be found in many tissues displaying a stronger presence in the liver kidney and pancreas indicating its significance in these organs. The enzyme contains a selenocysteine residue at its active site which is essential for its enzymatic activity.
Biological function summary

TXNRD1 functions as an integral part of the thioredoxin system which includes thioredoxin and NADPH. It maintains the redox homeostasis in cells by regenerating oxidized thioredoxin. This action allows thioredoxin to in turn reduce disulfide bonds in various substrate proteins. Furthermore TXNRD1 participates in regulating cellular processes such as DNA synthesis and repair signal transduction and antioxidant defense. It does not work alone but collaborates with various redox-active components to achieve its roles.

Pathways

TXNRD1 participates in the antioxidant defense and redox signaling pathways. Within the antioxidant defense pathway TXNRD1 along with thioredoxin provides resistance to oxidative stress. In the redox signaling pathway the protein interacts with NF-κB signaling processes impacting cell inflammation and apoptosis. These pathways are vital for cellular health and are connected through thioredoxin emphasizing the interconnected nature of TXNRD1-related processes.

TXNRD1 has associations with cancer and neurodegenerative diseases. Elevated levels of TXNRD1 have been observed in various cancers including lung and hepatocellular carcinoma suggesting a role in tumor growth and survival through redox regulation. Additionally TXNRD1 is linked to neurodegenerative diseases such as Alzheimer's where oxidative stress plays a contributory role. In these conditions TXNRD1 interacts closely with other redox proteins like glutathione reductase highlighting its potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed : 8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. A selenocysteine residue at the C-terminal active site is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed : 10849437).. Isoform 1. Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.. Isoform 4. Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.. Isoform 5. Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed : 15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed : 9774665).
See full target information TXNRD1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer research communications 4:1369-1379 PubMed38709075

2024

Pan-Cancer Interrogation of B7-H3 (CD276) as an Actionable Therapeutic Target Across Human Malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Carly D Miller,John R Lozada,Nicholas A Zorko,Andrew Elliott,Allison Makovec,Milan Radovich,Elisabeth I Heath,Neeraj Agarwal,Rana R Mckay,Rohan Garje,Bruno R Bastos,Dave S B Hoon,Jacob J Orme,Oliver Sartor,Ari VanderWalde,Chadi Nabhan,George Sledge,Eugene Shenderov,Scott M Dehm,Emil Lou,Jeffrey S Miller,Justin H Hwang,Emmanuel S Antonarakis
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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