AB76005

Anti-HSC70 Interacting Protein HIP antibody [EP3264]

RabMAb
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(3 제품이 사용된 논문 )

Rabbit Monoclonal HSC70 Interacting Protein HIP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human normal colon by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

All lanes:

Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005) at 1/100000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 47 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3264

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).

See full target information ST13

대체 명칭 보기

AAG2, FAM10A1, HIP, SNC6, ST13, Hsc70-interacting protein, Hip, Aging-associated protein 2, Progesterone receptor-associated p48 protein, Protein FAM10A1, Putative tumor suppressor ST13, Renal carcinoma antigen NY-REN-33, Suppression of tumorigenicity 13 protein

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 11:672684 PubMed34249713

2021

Exosomal miR-183-5p Shuttled by M2 Polarized Tumor-Associated Macrophage Promotes the Development of Colon Cancer Targeting THEM4 Mediated PI3K/AKT and NF-κB Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shangxin Zhang,Deguan Li,Min Zhao,Fei Yang,Changye Sang,Changhong Yan,Zhenjun Wang,Yongxiang Li

Biomaterials science 7:4310-4324 PubMed31410428

2019

Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine L Le Maitre

Biomedicine & pharmacotherapy = Biomedecine & phar 107:1210-1217 PubMed30257335

2018

Toll-like receptor 4 shRNA attenuates lipopolysaccharide-induced epithelial-mesenchymal transition of intrahepatic biliary epithelial cells in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shifang Tang,Xiuhan Jiang,Lang Wu,Shifa Chen,Ling Chen,Jichang Jiang,Pengzhan Yan,Fang Wang,Kui Tu,Dianbei Wang,Jin Gu,Lijin Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSC70 Interacting Protein HIP antibody [EP3264]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise