AB243881

Anti-hSET1/SET1 antibody [BLR024E]

Recombinant
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Rabbit Recombinant Monoclonal hSET1/SET1 antibody. Suitable for IHC-P, IP, WB, ICC and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SETD1A aa 1200-1250.

대체 명칭 보기

KIAA0339, KMT2F, SET1, SET1A, SETD1A, Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1/Ash2 histone methyltransferase complex subunit SET1, hSET1A

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human lung cancer labeling human hSET1 with ab243881 used at 1 : 25. Substrate : DAB. Nuclear staining on human lung cancer is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Supplier Data

Immunocytochemistry - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded human jurkat cells labeling human hSET1 with ab243881 used at 1 : 25. Substrate : DAB. Nuclear staining on jurkat cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human ovarian carcinoma labeling human hSET1 with ab243881 used at 1 : 25. Substrate : DAB. Nuclear staining on human ovarian carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Unknown

Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.

Lane 1 : ab243881 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

All lanes:

Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Predicted band size: 186 kDa

false

Unknown

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Western blot analysis using ab243881 at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate.

Lane 2 : HEK-293T whole cell lysate .

Lane3 : Jurkat whole cell lysate.

Lane 4 : TCMK-1 whole cell lysate.

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.

All lanes:

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Predicted band size: 186 kDa

false

Supplier Data

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

All lanes:

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

K-562 whole cell lysate at 50 µg

Lane 3:

U2OS whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Lane 5:

Hep-G2 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 186 kDa

Observed band size: 270 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

All lanes:

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

BW5147.3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 186 kDa

Observed band size: 270 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR024E

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human SETD1A aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.

O15047

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형

Liquid

Purification 관련 사항

Recombinant antibody was purified from cell culture supernatant.

보관 버퍼

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HSET1 also known as SET1 is a protein complex known for its role in histone methylation specifically on histone H3 at lysine 4 (H3K4). This methylation activity plays an important role in gene expression regulation. Predominantly expressed in the nucleus of eukaryotic cells hSET1 forms part of the COMPASS complex which weighs around 600 kDa. The enzyme encompasses various subunits each contributing to the overall stability and functionality of the complex.

Biological function summary

HSET1 influences chromatin structure modifications that regulate transcriptional activity. As a histone methyltransferase its activity results in epigenetic changes that turn genes on or off. hSET1 as part of the COMPASS complex recruits other factors to transcription initiation sites leading to active gene expression. This activity highlights its significant role in cell differentiation and development affecting how cells progress from one state to another.

Pathways

HSET1 plays a major role in the regulation of the Wnt signaling pathway and the DNA damage response pathway. Through the Wnt pathway hSET1 influences the dynamic process of cellular growth and differentiation interacting with proteins such as β-catenin. In the DNA damage response hSET1 modulates chromatin modifications facilitating the repair of damaged DNA coordinating with proteins like p53 to maintain genomic stability.

Aberrations in hSET1 functions associate with leukemias and certain neurodevelopmental disorders. In leukemia inappropriate activity or mutation in hSET1 leads to misregulated gene expression contributing to malignancy progression. Neurodevelopmental disorders have also shown correlations with abnormal hSET1 expression possibly affecting synaptic connectivity. Proteins such as EZH2 in the context of leukemia show interactions with SET1-related pathways highlighting its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism (PubMed : 12670868, PubMed : 25561738). Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 29937342, PubMed : 31197650, PubMed : 32346159). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (By similarity) (PubMed : 31197650). Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation (PubMed : 29937342, PubMed : 32346159). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed : 12670868). Binds RNAs involved in RNA processing and the DNA damage response (PubMed : 38003223).

See full target information SETD1A

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular psychiatry 30:5680-5693 PubMed40962831

2025

Mutations of schizophrenia risk gene SETD1A dysregulate synaptic function in human neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Su,Hanwen Zhang,Yan Hong,Qian Yang,Le Wang,Tiffany Le,Jiayi Liu,Lasya Cheruvu,Emily Labour,Siwei Zhang,Karla Mendez-Maldonado,Anat Kreimer,Hongjun Song,Guo-Li Ming,Jubao Duan,Zhiping P Pang

Cancer cell international 24:397 PubMed39639311

2024

Ailanthone disturbs cross-talk between cancer cells and tumor-associated macrophages via HIF1-α/LINC01956/FUS/β-catenin signaling pathway in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xubin Deng,Qianbing Zhang,Fa Jin,Fengfei Lu,Guosheng Duan,Luwei Han,Meiling Zhu,Zhengyan Yang,Gong Zhang

Thoracic cancer 14:2579-2590 PubMed37548102

2023

SETD1A-mediated H3K4me3 methylation upregulates lncRNA HOXC-AS3 and the binding of HOXC-AS3 to EP300 and increases EP300 stability to suppress the ferroptosis of NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenliang Shi,Hao Zhang,Yimeng Shen,Sipei Zhang,Xun Zhang,Yijun Xu,Daqiang Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hSET1/SET1 antibody [BLR024E]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunocytochemistry - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

Western blot - Anti-hSET1/SET1 antibody [BLR024E] (AB243881)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise