Anti-Hsp27 (phospho S82) antibody [EPR7278]
- RabMAb
- Recombinant
- 각 태그의 뜻
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(11 제품이 사용된 논문 )
Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 11 publications.
대체 명칭 보기
HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27
- IP
Lab
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)
Predicted band size: 23 kDa
false
- IP
Lab
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)
Predicted band size: 23 kDa
false
- WB
Lab
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Blocking and diluting buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates at 20 µg
Lane 2:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
false
Exposure time: 1min
- WB
Lab
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution
All lanes:
HeLa cell lysate treated with heat shock (44°C) at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
false
- WB
Lab
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution
All lanes:
HeLa cells treated with heat shock (44°C) at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa,89 kDa
Observed band size: 27 kDa,80 kDa
false
- WB
Unknown
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution
Lane 1:
Untreated HeLa cell lysate at 10 µg
Lane 2:
HeLa cell lysate treated with heat shock (44°C) at 10 µg
Secondary
All lanes:
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa,216 kDa,23 kDa,28 kDa,286 kDa
Observed band size: 15 kDa,260 kDa,48 kDa
false
- WB
Lab
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution
Lane 1:
Mouse heart at 10 µg
Lane 2:
Rat heart at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
false
- WB
Lab
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution
Lane 1:
Mouse heart at 10 µg
Lane 2:
Rat heart at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
false
- Dot
Lab
Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987)
Dot blot analysis of Lane 1 : Hsp27 (pS82) phospho peptide and Lane 2 : Hsp27 non-phospho peptide labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051 a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time : 10 seconds.
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
제품 프로토콜
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타겟 정보
제품이 사용된 논문 (11)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Journal of cancer research and clinical oncology 151:264 PubMed40971071
2025
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Cell death & disease 16:470 PubMed40593489
2025
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MedComm 6:e70032 PubMed40013315
2025
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Journal of Cancer 15:645-658 PubMed38213722
2024
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Nature cell biology 25:467-480 PubMed36690850
2023
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Molecules (Basel, Switzerland) 25: PubMed32570757
2020
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International journal of nanomedicine 15:3291-3302 PubMed32494130
2020
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Translational cancer research 8:1268-1278 PubMed35116869
2019
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Clinical and experimental immunology 194:137-152 PubMed30014472
2018
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In vivo (Athens, Greece) 32:637-642 PubMed29695571
2018
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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