JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242006

Anti-Hsp47 antibody [1C4-1A6]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Mouse Monoclonal Hsp47 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINH1.

대체 명칭 보기

CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)

2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (green, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.

Primary incubation for 12 hours at 4°.

Secondary antibody : FITC Goat Anti-Mouse (green) at 1 : 200 for 2 hours at RT.

Cell were heat shocked at 42°C for 1 hour.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)

2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (red, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.

Primary incubation for 12 hours at 4°.

Secondary antibody : APC Goat Anti-Mouse (red) at 1 : 200 for 2 hours at RT.

Cell were heat shocked at 42°C for 1 hour.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

1C4-1A6

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SERPINH1.

P50454

style
left-column

대체품

Primary Antibodies

AB86750

Anti-Hsp47 antibody [134CT7.1.8]

KO Validated

Western blot - Anti-Hsp47 antibody [134CT7.1.8] (AB86750)

  • 1
  • 1
Primary Antibodies

AB109117

Anti-Hsp47 antibody [EPR4217]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (AB109117)

  • 5
  • 8
Primary Antibodies

AB226052

Anti-Hsp47 antibody [EPR4217] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] - BSA and Azide free (AB226052)

  • 0
  • 0

보완 제품

Assay Kits

AB108861

Human Heat Shock Protein 47 ELISA Kit (SERPINH1)

Sandwich ELISA - Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (AB108861)

  • 0
  • 0
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

  • 4
  • 24
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from TCS.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.
Biological function summary

Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.

Pathways

Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.

Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
See full target information SERPINH1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com