Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker
- 20ul selling size
- RabMAb
- Recombinant
- Lab Essentials
- 각 태그의 뜻
4
(4 리뷰들)
|
(34 제품이 사용된 논문 )
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.
The nuclear counter stain is DAPI (blue). COX IV is detected with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) (red) at 1/1000 dilution.
The negative controls are as follows : -
-ve control 1 : ab190828 at 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution.
-ve control 2 : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Hsp60 with ab190828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular and cytoplasmic staining on human liver (PMID : 18548335). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embyro fibroblast cell line) cell line labeling Hsp60 with ab190828 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Hsp60 with ab190828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular and cytoplasmic staining on rat liver (PMID : 18548335). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Supplier Data
Immunoprecipitation - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Hsp60 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) cell lysate with ab190828 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190828 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : NIH/3T3 cell lysate 10 μg (Input).
Lane 2 : ab190828 IP in NIH/3T3 cell lysate .
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190828 in NIH/3T3 cell lysate .
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.
All lanes:
Immunoprecipitation - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828)
Predicted band size: 61 kDa
false
- WB
Supplier Data
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Exposure time : Lanes 1,2 and 3 : 3 minutes; Lane 4 : 1 minute.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/1000 dilution
Lane 1:
Human brain lysate at 10 µg
Lane 2:
Human fetal heart lysate at 10 µg
Lane 3:
Human fetal kidney lysate at 10 µg
Lane 4:
Human fetal spleen lysate at 10 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
true
- WB
Supplier Data
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Exposure time : Lanes 1,2 and 3 : 30 seconds; Lane 4 : 15 seconds.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/5000 dilution
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 2:
NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 20 µg
Lane 3:
Neuro-2a (mouse neuroblastoma cell line) cell lysate at 20 µg
Lane 4:
C6 (rat glial tumor cell line) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
true
- WB
Supplier Data
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
Exposure time : Lane 1 : 30 seconds; Lane 2 : 1 minute; Lanes 3,4 and 5 : 10 seconds; Lane 6 : 5 seconds.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/1000 dilution
Lane 1:
Mouse heart lysate at 10 µg
Lane 2:
Mouse spleen lysate at 10 µg
Lane 3:
Rat brain lysate at 10 µg
Lane 4:
Rat heart lysate at 10 µg
Lane 5:
Rat spleen lysate at 10 µg
Lane 6:
Rat liver lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
true
관련 conjugated 항체와 다양한 조성의 항체 (4)
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Anti-Hsp60 antibody [EPR18245-93] - BSA and Azide free (Capture)
-
Anti-Hsp60 antibody [EPR18245-93] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker
Reactivity 정보
제품 세부 정보
What is this antibody validated in?
Anti-Hsp60 antibody [EPR18245-93] (ab190828) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Hsp60?
Anti-Hsp60 [EPR18245-93] (ab190828) specifically detects a band for Hsp60 (UniProt: P63038) at a molecular weight of 61kDa.
Trusted by the scientific community
Anti-Hsp60 [EPR18245-93] (ab190828) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.
Pathways
Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.
제품 프로토콜
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타겟 정보
대체 명칭 보기
제품이 사용된 논문 (34)
Recent publications for all applications. Explore the 전체 목록and refine your search
Molecular medicine reports 32: PubMed40999952
2025
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Acta pharmaceutica Sinica. B 15:3125-3148 PubMed40654366
2025
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CNS neuroscience & therapeutics 31:e70394 PubMed40237297
2025
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Bone research 13:47 PubMed40229258
2025
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Antioxidants (Basel, Switzerland) 14: PubMed40227213
2025
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International journal of molecular sciences 25: PubMed39684599
2024
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Communications biology 7:1229 PubMed39354016
2024
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Materials today. Bio 28:101235 PubMed39318374
2024
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Frontiers in oncology 14:1415762 PubMed39224812
2024
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Theranostics 14:4278-4296 PubMed39113800
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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