AB216634

Anti-Hsp60 antibody - Mitochondrial Marker

Lab Essentials
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal Hsp60 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HSPD1 aa 500-600 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling Hsp60 with ab216634 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Hsp60 with ab216634 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Hsp60 with ab216634 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (ab216634) at 1/300 dilution

All lanes:

MCF7 lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (ab216634) at 1/300 dilution

All lanes:

mouse kidney lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (ab216634) at 1/300 dilution

All lanes:

mouse heart lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (ab216634) at 1/300 dilution

All lanes:

mouse liver lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 61 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human HSPD1 aa 500-600 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10809

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.

Biological function summary

The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.

Pathways

Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.

Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

See full target information HSPD1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 21:817 PubMed37974228

2023

HOXD9/miR-451a/PSMB8 axis is implicated in the regulation of cell proliferation and metastasis via PI3K/AKT signaling pathway in human anaplastic thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhong,Fan Yu,Ling Yang,Yu Wang,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Shiyang Sheng,Zhongwei Lv,Li Weng,Bo Wu,Xiaoping Zhang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp60 antibody - Mitochondrial Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB216634)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise