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AB6514

Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

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(5 리뷰들)

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(72 제품이 사용된 논문 )

Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) is a mouse monoclonal antibody detecting HSV 1 ICP4 Immediate Early Protein in Western Blot, ICC/IF, ELISA. Suitable for Herpes simplex virus.

- Over 60 publications
- Trusted since 2002
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • ICC/IF

AbReview5375****

Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • WB

AbReview8120****

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

All lanes:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

Lane 1:

HeLa cells (whole cell lysate) - mock infection, cytosolic fraction at 10 µg

Lane 2:

HeLa cells (whole cell lysate) - mock infection, membrane fraction at 10 µg

Lane 3:

HeLa cells (whole cell lysate) - mock infection, nuclear fraction at 10 µg

Lane 4:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction at 10 µg

Lane 5:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction at 10 µg

Lane 6:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction at 10 µg

Lane 7:

HeLa cells (whole cell lysate) - mock infected at 10 µg

Lane 8:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours at 10 µg

Secondary

All lanes:

HRP conjugated sheep anti-mouse IgG

Observed band size: 97 kDa

false

This image is courtesy of an anonymous Abreview

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • WB

Supplier Data

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

Lane 1:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution

Lane 2:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution

Lane 3:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution

Lane 4:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution

All lanes:

BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract at 10 µg

Secondary

All lanes:

Anti-Mouse secondary at 1/5000 dilution

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

10F1

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Herpes simplex virus

Applications

WB, ELISA, ICC/IF

applications

Specificity

<p>ELISA titer 1/25,600 absorption of 0&#46;10</p>

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보완 제품

Primary Antibodies

AB6513

Anti-HSV1 ICP0 antibody [5H7]

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP0 antibody [5H7] (AB6513)

  • 5
  • 1
Primary Antibodies

AB20194

Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2]

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)

  • 5
  • 1
Primary Antibodies

AB110226

Anti-HSV1 + HSV2 VP16 antibody [LP1]

Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

  • 0
  • 0
Primary Antibodies

AB6506

Anti-HSV1 + HSV2 gB antibody [10B7]

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 gB antibody [10B7] (AB6506)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Herpes simplex virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/800", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/25600", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Herpes simplex virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Herpes simplex virus samples.

Trusted by the scientific community
Anti-HSV 1 ICP4 Immediate Early Protein [10F1] (ab6514) was first used in a scientific publication in 2002 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-HSV 1 ICP4 Immediate Early Protein [10F1] (ab6514) has over 5 independent reviews from customers.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The HSV 1 ICP4 Immediate Early Protein also known as Infected Cell Protein 4 (ICP4) has a significant mechanical role during the herpes simplex virus type 1 (HSV-1) infection. ICP4 has a molecular mass of approximately 175 kDa. It uniquely expresses in the early phase of HSV-1 infection in the host cells primarily in the nucleus where it functions as a transcriptional activator. Its expression tightly regulates the viral replication cycle making it an essential protein for the virus.
Biological function summary

HSV-1 ICP4 plays a critical role as a major regulator of viral gene expression. ICP4 acts within a transcriptional complex where it binds to specific DNA sequences to activate the transcription of early and late viral genes. The protein can directly interact with host transcription machinery modifying the host cell's transcriptional dynamics. This ability allows the virus to hijack cellular machinery efficiently advancing its replication and further propagation within the host.

Pathways

HSV-1 ICP4 integrates into key transcriptional pathways that govern viral gene expression. The protein is pivotal in regulating both the early and late gene expression pathways of HSV-1 establishing a coordinated effort for viral replication. ICP4 associates with other viral proteins like ICP0 and ICP27 which function collaboratively within these pathways to modulate the synthesis of viral DNA and proteins necessary for successful virus assembly and release.

HSV-1 ICP4 strongly links to conditions such as herpes simplex virus infection and associated complications including encephalitis. During infection ICP4 contributes to the pathogenesis by promoting viral replication and persistence in host cells. Its interaction with the protein ICP27 further associates with the inhibition of host cell apoptosis sustaining viral survival and enhancing infection severity. These connections highlight its relevance in antiviral research and therapy development where targeting ICP4 could mitigate HSV-1-related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.
See full target information ICP4

대체 명칭 보기

IE175, RS1, ICP4, Major viral transcription factor ICP4, Alpha-4 protein, Infected cell protein 4, Transcriptional activator IE175
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제품이 사용된 논문 (72)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of virology 99:e0127625 PubMed40874743

2025

Camptothecin, a topoisomerase I inhibitor, impedes productive herpes simplex virus type 1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Heath,Alison E Lloyd,Raegen M Kulinski,Daniel P Fromuth,Jill A Dembowski

Molecular therapy. Methods & clinical development 33:101471 PubMed40454417

2025

Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofei E,Benjamin Wright,Brian E Collins,Lauren Peters,Sviatlana Rose,Peter Webster,Carl A Morris,Sharon McGonigle

Neurobiology of disease 206:106804 PubMed39818277

2025

Phosphorylated-tau associates with HSV-1 chromatin and correlates with nuclear speckles decondensation in low-density host chromatin regions.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo D'Aiuto,Jill K Caldwell,Terri G Edwards,Chaoming Zhou,Matthew L McDonald,Roberto Di Maio,Wood A Joel,Vanesa R Hyde,Callen T Wallace,Simon C Watkins,Maribeth A Wesesky,Or A Shemesh,Vishwajit L Nimgaonkar,David C Bloom

PLoS pathogens 20:e1012621 PubMed39374265

2024

The HSV-1 encoded CCCTC-binding factor, CTRL2, impacts the nature of viral chromatin during HSV-1 lytic infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Singh,Liqian Zhu,Mason A Shipley,Ziyun A Ye,Donna M Neumann

Biomolecules 14: PubMed38786010

2024

Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Salgado,Beatriz Izquierdo,Alba Zapata,Isabel Sastre,Henrike Kristen,Julia Terreros,Víctor Mejías,María J Bullido,Jesús Aldudo

Antiviral research 225:105870 PubMed38556059

2024

Glucocorticoid receptor and specificity protein 1 (Sp1) or Sp3, but not the antibiotic Mithramycin A, stimulates human alphaherpesvirus 1 (HSV-1) replication.

Applications

Unspecified application

Species

Unspecified reactive species

Fouad S El-Mayet,Vanessa Claire Santos,Nishani Wijesekera,Sydney Lubbers,Kelly S Harrison,Hafez Sadeghi,Clinton Jones

Nature communications 15:1991 PubMed38443365

2024

Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Deng,Yuqi Lin,Siyu Chen,Yuhang Xiang,Hongjia Chen,Shuyuan Qi,Hyung Suk Oh,Biswajit Das,Gloria Komazin-Meredith,Jean M Pesola,David M Knipe,Donald M Coen,Dongli Pan

EMBO reports 24:e56327 PubMed37846490

2023

CCR4-NOT differentially controls host versus virus poly(a)-tail length and regulates HCMV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Burgess,Rebecca Grande,Sofia Riccio,Ikshitaa Dinesh,Gerlof Sebastiaan Winkler,Daniel P Depledge,Ian Mohr

Journal of virology 97:e0130523 PubMed37823644

2023

Impaired glucocorticoid receptor function attenuates herpes simplex virus 1 production during explant-induced reactivation from latency in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly S Harrison,Nishani Wijesekera,Anastasia G J Robinson,Vanessa C Santos,Robert H Oakley,John A Cidlowski,Clinton Jones

PLoS pathogens 19:e1011539 PubMed37486931

2023

Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica E Packard,Maya R Williams,Daniel P Fromuth,Jill A Dembowski
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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