Anti-HtrA1 antibody [HL3878] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HtrA1 antibody [HL3878] - BSA and Azide free (AB327514)

ab327514 detects HtrA1 protein by immunofluorescent analysis.
Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : HtrA1 stained by ab327514 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA1 antibody [HL3878] - BSA and Azide free (AB327514)

ab327514 detects HtrA1 protein by immunohistochemical analysis.
Sample : Transfected 293T cell FFPE Cell Pellet Blocks.
Green : HtrA1 stained by ab327514 diluted at 1 : 1000.
Red : DDDDK tag antibody diluted at 1 : 1000.
Blue : Fluoroshield with DAPI .
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

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Western blot - Anti-HtrA1 antibody [HL3878] - BSA and Azide free (AB327514)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327514 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-HtrA1 antibody [HL3878] - BSA and Azide free (ab327514) at 1/1000 dilution

Lane 1:

SK-N-SH whole cell extracts at 30 µg

Lane 2:

IMR32 whole cell extracts at 30 µg

Lane 3:

SK-N-AS whole cell extracts at 30 µg

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