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AB316358

Anti-Human IgA antibody [15D6]

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Mouse Monoclonal IGHA2 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human IGHA2.

대체 명칭 보기

Immunoglobulin heavy constant alpha 2, Ig alpha-2 chain C region, Ig alpha-2 chain C region BUT, Ig alpha-2 chain C region LAN, IGHA2

2 이미지
Flow Cytometry - Anti-Human IgA Antibody [15D6] (AB316358)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human IgA Antibody [15D6] (AB316358)

Blue line : Flow cytometric analysis of human peripheral blood leukocytes. Primary incubation 1hr (1 : 50-1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Fluorescein Isotype control.

Western blot - Anti-Human IgA Antibody [15D6] (AB316358)
  • WB

Supplier Data

Western blot - Anti-Human IgA Antibody [15D6] (AB316358)

(0.01 µg/ml) staining in human plasma (A) and serum (B) (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Human IgA Antibody [15D6] (ab316358) at 0.01 µg/mL

Lane 1:

Human plasma

Lane 2:

Human serum

true

Exposure time: 1hr

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

15D6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Native Full Length Protein corresponding to Human IGHA2.

P01877

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보완 제품

Assay Kits

AB315787

Human IgA2 ELISA Kit

SimpleStep

Sandwich ELISA - Human IgA2 ELISA Kit (AB315787)

  • 0
  • 0
Primary Antibodies

AB7400

Anti-Human IgA antibody [3B7]

  • 0
  • 0
Primary Antibodies

AB106731

Anti-IgA antibody [KT41]

Sandwich ELISA - Anti-IgA antibody [KT41] (AB106731)

  • 0
  • 0
Secondary Antibodies

AB97014

Goat Anti-Mouse IgA alpha chain (DyLight® 650)

  • 0
  • 0
Secondary Antibodies

AB96871

Goat Anti-Mouse IgG H&L (DyLight® 488)

Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) (AB96871)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
보관 버퍼
Preservative: 0.1% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1 month
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Human IgA also known as Immunoglobulin A is an antibody that plays a critical role in the immune system. It weighs approximately 160 kDa and is predominantly found in mucosal areas such as the respiratory and gastrointestinal tracts as well as in saliva tears and breast milk. In its molecular form IgA often exists as a dimer two IgA molecules linked by a joining (J) chain. The primary function of IgA is to protect mucosal surfaces by neutralizing pathogens and toxins preventing them from adhering to epithelial cells.
Biological function summary

IgA contributes to the immune defense by participating in immune exclusion mechanisms. As part of this process IgA interacts with the polymeric immunoglobulin receptor (pIgR) to transport across epithelial cells making it important for mucosal immunity. IgA is also connected with secretory components that further stabilize and enhance its longevity in mucosal secretions. It plays an integral role in forming complexes that assist in eliminating antigens from mucosal surfaces.

Pathways

Human IgA is an important player in the mucosal immune response pathway defending against pathogens by zeroing in on antigen neutralization. Its interactions with the pIgR are central to the transport mechanism through epithelial cells where the IgA-pIgR complex becomes the secretory IgA. This pathway shares a strong connection with related proteins such as IgM another immunoglobulin involved in the early stages of immune response and found in serum.

IgA is associated with IgA nephropathy a kidney disorder caused by the deposition of IgA in the kidneys leading to inflammation and functional impairment. Additionally IgA plays a role in celiac disease where IgA antibodies are produced in response to gluten and can contribute to intestinal damage. In these conditions the relationship between IgA and other proteins like complement component 3 (C3) is significant as improper regulation of these pathways may lead to tissue damage and disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).
See full target information IGHA2
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Product promise

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