AB322958

Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA]

Recombinant
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Mouse Recombinant Monoclonal Minor capsid protein L2 antibody. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human papillomavirus 4, Recombinant fragment - Human papillomavirus 4, Transfected cell lysate - Human papillomavirus 4 samples.

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Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HPV4 L2 with ab322958 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 4 L2 and Human papillomavirus type 65 L2 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a human papillomavirus type 60 L2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

Indirect ELISA analysis of ab322958 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human papillomavirus 4 Minor capsid protein L2.

Antigen concentration : 5000 ng/ml

Supplier Data

Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Human papillomavirus type 4 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T transfected with a Human papillomavirus type 65 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 4:

293T transfected with a Human papillomavirus type 60 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 70s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HPV4L2(20-38)_4SA

Isotype

IgG1

Carrier free

Reacts with

Human papillomavirus 4

Applications

I-ELISA, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross-react with overexpressed human papillomavirus type 4 L2 protein and human papillomavirus type 65 L2 protein.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus 4": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human papillomavirus 4": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human papillomavirus 4": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human papillomavirus 4": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.

See full target information L2

대체 명칭 보기

Minor capsid protein L2, Minor capsid protein L2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA]

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise