Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free (AB324763)

This data was developed using ab322235, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a Human respiratory syncytial virus Fusion (F) Glycoprotein expression vector containing a His-tag cell pellet; (B) HEK-293T transfected with an empty vector containing a His-tag cell pellet labeling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a Human respiratory syncytial virus Fusion (F) Glycoprotein expression vector containing a His-tag cell pellet. No staining on (B) HEK-293T transfected with an empty vector containing a His-tag cell pellet.
The section was incubated with ab322235 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free (AB324763)

This data was developed using ab322235, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human lung.
The section was incubated with ab322235 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free (AB324763)

This data was developed using ab322235, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/2000 (0.56 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free (AB324763)

This data was developed using ab322235, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322235 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human respiratory syncytial virus (RSV) (B, strain 18537) Fusion glycoprotein, Human respiratory syncytial virus (RSV) (A, strain RSS-2) Fusion glycoprotein.

Antigen concentration : 5000 ng/ml