AB70744

Anti-HURP antibody

Name: Anti-HURP antibody
Brand: Abcam Limited
SKU: AB70744
Rating: 5 (3 reviews)

(3 리뷰들)

(10 제품이 사용된 논문 )

Rabbit Polyclonal HURP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DLGAP5.

2 이미지

Unknown

Immunoprecipitation - Anti-HURP antibody (AB70744)

Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-HURP antibody (ab70744)

Predicted band size: 95 kDa

false

Unknown

Western blot - Anti-HURP antibody (AB70744)

All lanes:

Western blot - Anti-HURP antibody (ab70744) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 95 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DLGAP5. The exact immunogen used to generate this antibody is proprietary information.

Q15398

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HURP also known as Hepatoma Up-Regulated Protein is a protein involved in spindle formation during cell division. It has an approximate mass of 82 kilodaltons. The expression of HURP is notably high in the liver and is also present in various other tissues. It performs essential mechanical roles in stabilizing microtubules which are necessary for accurate chromosome segregation during mitosis.

Biological function summary

Hepatoma Up-Regulated Protein plays an important role in the mitotic spindle assembly. As a part of the chromosomal passenger complex it works alongside other proteins to facilitate efficient cell cycle progression. HURP localizes to the mitotic microtubules and is particularly active during the metaphase of cell division contributing to correct chromosome alignment and spindle attachment.

Pathways

Hepatoma Up-Regulated Protein functions within the cell cycle regulation pathway especially influencing mitotic processes. The intricate network of proteins it associates with includes Aurora kinase B and Ran GTPase which are essential for mitotic checkpoint control and spindle assembly. HURP helps maintain chromosomal stability which is important for preventing genetic aberrations.

Hepatoma Up-Regulated Protein has links to several cancer types including liver cancer. HURP overexpression often correlates with tumor progression and poor prognosis. It interacts with proteins like Aurora kinase B which is also implicated in oncogenesis suggesting that HURP may serve as a potential biomarker or therapeutic target in cancer research.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

See full target information DLGAP5

대체 명칭 보기

DLG7, KIAA0008, DLGAP5, Disks large-associated protein 5, DAP-5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein, HURP

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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:8844 PubMed39397030

2024

HURP binding to the vinca domain of β-tubulin accounts for cancer drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Athira Saju,Po-Pang Chen,Tzu-Han Weng,Su-Yi Tsai,Akihiro Tanaka,Yu-Ting Tseng,Chih-Chia Chang,Chun-Hsiung Wang,Yuta Shimamoto,Kuo-Chiang Hsia

Scientific reports 13:17282 PubMed37828032

2023

LINC01343 targets miR-526b-5p to facilitate the development of hepatocellular carcinoma by upregulating ROBO1.

Applications

Unspecified application

Species

Unspecified reactive species

Song Wu,Tao Tang,Hongchi Zhou,Jing Huang,Xiaoliang Kang,Junli Zhang

Experimental and therapeutic medicine 26:480 PubMed37745040

2023

Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Luo,Yumei Zhong,Yanding Guo,Jingwei Nie,Yimei Xu,Haiyan Zhou

Frontiers in cell and developmental biology 11:981425 PubMed37484914

2023

HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue.

Applications

Unspecified application

Species

Unspecified reactive species

Stylianos Didaskalou,Christos Efstathiou,Sotirios Galtsidis,Ilοna Kesisova,Aliaksandr Halavatyi,Tountzai Elmali,Avgi Tsolou,Andreas Girod,Maria Koffa

Cancer biomarkers : section A of Disease markers 39:1-13 PubMed37334578

2023

circ_0058063 promotes breast cancer progression by upregulating DLGAP5 via sponging miR-557.

Applications

Unspecified application

Species

Unspecified reactive species

Kongjun Zhu,Cen Yi,Chuanming Tong

Bioengineered 15:1996016 PubMed34699315

2021

Retracted article: MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changwen Li,Sen Peng,Chuangang Tang

OncoTargets and therapy 12:10057-10075 PubMed31819501

2019

Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Manfei Si,Junji Zhang,Jianzhong Cao,Zhibo Xie,Shan Shu,Yapei Zhu,Jinghe Lang

Biomedicine & pharmacotherapy = Biomedecine & phar 111:813-820 PubMed30616080

2019

In vitro study of anti-ER positive breast cancer effect and mechanism of 1,2,3,4-6-pentyl-O-galloyl-beta-d-glucose (PGG).

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Xiang,Juan Tang,Qin Luo,Jinfeng Xue,Yexing Tao,Honghui Jiang,Jing Tian,Caiwen Fan

The Journal of biological chemistry 293:15733-15747 PubMed30054275

2018

The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Zhang,Lora Tan,Qiaoyun Yang,Chenyu Li,Yih-Cherng Liou

Biology open 6:463-470 PubMed28228376

2017

Kif18A is a highly processive kinesin required for meiotic spindle integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Martin M Möckel,Andreas Heim,Thomas Tischer,Thomas U Mayer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HURP antibody

Immunoprecipitation - Anti-HURP antibody (AB70744)

Western blot - Anti-HURP antibody (AB70744)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (10)

Product promise