AB237521

Anti-HYPE antibody

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Rabbit Polyclonal HYPE antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FICD aa 50-200.

대체 명칭 보기

HIP13, HYPE, UNQ3041/PRO9857, FICD, Protein adenylyltransferase FICD, AMPylator FICD, De-AMPylase FICD, FIC domain-containing protein, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E, HIP-13

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)

Paraffin-embedded human kidney tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)

Paraffin-embedded human small intestine tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-HYPE antibody (AB237521)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HYPE antibody (AB237521)

PC-3 (Human prostate adenocarcinoma cell line) cells stained for HYPE (green) using ab237521 at 1/166 dilution in ICC/IF. Secondary antibody is an Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FICD aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BVA6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity greater than 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HYPE also known as RHBDF1 is an endoplasmic reticulum protein that functions as a membrane protein involved in protein regulation and trafficking. It has an approximate mass of 38 kDa. HYPE is expressed widely within various tissues but shows significant levels in cells of the liver and kidney. This protein plays a role in multiple cellular processes reflecting its versatile functionality.

Biological function summary

This protein impacts endoplasmic reticulum stress responses and protein homeostasis. It does not seem to form part of a larger complex but operates independently to regulate the degradation of specific proteins aiding in maintaining cellular balance. HYPE's actions contribute to preventing protein aggregation that could disrupt cellular function.

Pathways

HYPE participates in the unfolded protein response (UPR) and the ER-associated degradation (ERAD) pathway. It acts alongside proteins involved in protein folding and trafficking such as BiP and calnexin to ensure synthesis and processing occur efficiently. These pathways highlight HYPE’s interaction with mechanisms that manage protein quality control.

This protein has connections to liver fibrosis and kidney dysfunction. Its regulation of protein folding is significant in these diseases where improper protein management can lead to cellular damage. Researchers have identified its interaction with proteins like TGF-beta in the context of fibrosis linking its importance to disease progression and providing potential therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (PubMed : 36136088). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed : 25601083, PubMed : 36136088). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed : 19362538, PubMed : 25601083).

See full target information FICD

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 51: PubMed38624012

2024

Abscisic acid signaling through LANCL2 and PPARγ induces activation of p38MAPK resulting in dormancy of prostate cancer metastatic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Keshab Raj Parajuli,Younghun Jung,Russell S Taichman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HYPE antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)

Immunocytochemistry/ Immunofluorescence - Anti-HYPE antibody (AB237521)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise