Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on Kupffer cells in the human liver. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on microglia in the human cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt

Lab

Flow Cytometry - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% Paraformaldehyde fixed, 90% Methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) / THP-1 (Human monocytic leukemia monocyte) cells labelling Iba1 with ab289874 at 1/1000 compared with a Goat IgG /Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Donkey anti-Goat IgG (Alexa Fluor^®488, ab150133) at 1/2000 was used as the secondary antibody.

Negative control :  MCF7 (PMID : 30386176).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND®RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling Iba1 with ab289874 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor^®488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic and membranous staining in THP-1 cell line. Negative control : MCF7 (PMID : 30386176).

was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor^®488) (ab150129) at 1/500 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on microglia in the rat cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on microglia in the mouse cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab289874).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Iba1 with ab289874 at 1/1000 dilution , followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) antibody at 1/500 dilution (Green).

Confocal image showing positive staining in mouse primary microglial cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

Rabbit anti-Iba1 antibody was used to counterstain Iba1 at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) at 1/500 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on Kupffer cells in the rat liver. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab289874).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Iba1 with ab289874 at 1/1000 dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) antibody at 1/500 dilution (Green).

Confocal image showing positive staining in rat primary microglial cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

Rabbit anti-Iba1 antibody was used to counterstain Iba1 at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) at 1/500 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) - BSA and Azide free (AB289876)

This data was developed using ab289874, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Iba1 with ab289874 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on Kupffer cells in the mouse liver.

The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.