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AB289370

Anti-Iba1 antibody [HL22] - BSA and Azide free

3

(2 리뷰들)

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1.
7 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunofluorescence analysis of THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Iba1 protein at cytoplasm using ab289370 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Iba1 using ab289370 diluted at 1 : 100 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labelling Iba1 protein with ab289370 at a 1/200 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue stained for Iba1 using ab289370 diluted at 1 : 1000 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of frozen mouse brain tissue stained for Iba1 using ab289370 diluted at 1 : 100 (green). Iba1 antibody [HL22-RT] diluted at 1 : 100 (red).

Nuclear stain is DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of frozen mouse spinal cord tissue stained for Iba1 using ab289370 diluted at 1 : 200 (green).

Nuclear stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue stained for Iba1 using ab289370 diluted at 1 : 1000 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL22

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human AIF1. The exact immunogen used to generate this antibody is proprietary information.

P55008

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보완 제품

Sample Prep & Detection Kits

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HRP Conjugation Kit - Lightning-Link®

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Sample Prep & Detection Kits

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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
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Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

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Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
Proteins & Peptides

AB114149

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Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

  • 0
  • 0
Primary Antibodies

AB209064

Anti-TMEM119 antibody [28-3] - Microglial marker

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Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (AB209064)

  • 4
  • 23
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
See full target information AIF1

대체 명칭 보기

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neural regeneration research 20:2611-2623 PubMed39314167

2024

High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mengshi Yang,Miao Bai,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Hao Li,Yu Deng,Hongbin Wu,Xiaojian Xu,Fei Niu,Xinlong Dong,Bin Zhang,Baiyun Liu

Nature communications 15:7855 PubMed39245680

2024

Human neural stem cell-derived artificial organelles to improve oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Wang,Mengke Zhao,Meina Wang,Dong Fu,Lin Kang,Yu Xu,Liming Shen,Shilin Jin,Liang Wang,Jing Liu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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