Anti-ICAM1 antibody [1A29]

• Flow Cyt

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Flow Cytometry - Anti-ICAM1 antibody [1A29] (AB171123)

Flow cytometry analysis of ICAM1 in Raji cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

• ICC

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Immunocytochemistry - Anti-ICAM1 antibody [1A29] (AB171123)

Immunocytochemical analysis of A439 cells staining ICAM1 with ab171123 at 1ug/ml.

Cells were fixed with 4% paraformaldehyde for 15 minutes, permeablilized with 0.25% Trition X-100 for 10 minutes and blocked with 5% BSA for 1 hour at room temparature. Cells were incubated with primary antibody at room temperature for 3 hours. Goat Anti-Mouse IgG (H&L) (Alexa Fluor® 488) was used as a secondary antibody.

• Flow Cyt

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Flow Cytometry - Anti-ICAM1 antibody [1A29] (AB171123)

Flow cytometry analysis of ICAM1 in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [1A29] (AB171123)

Immunohistochemical analysis of deparaffinized mouse kidney tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

• Flow Cyt

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Flow Cytometry - Anti-ICAM1 antibody [1A29] (AB171123)

Flow cytometry analysis of ICAM1 in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [1A29] (AB171123)

Immunohistochemical analysis of deparaffinized mouse spleen tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [1A29] (AB171123)

Immunohistochemical analysis of deparaffinized mouse lung tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

• WB

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Western blot - Anti-ICAM1 antibody [1A29] (AB171123)

ICAM1 western blot using anti-ICAM1 antibody [1A29] ab171123. Publication image and figure legend from Zhang, Y., Huo, W., et al., 2019, PLoS One, PubMed 31442237.

ab171123 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab171123 please see the product overview.

The results of RT-qPCR assay and Western analysis showed that si-NgBR down-regulated ICAM-1 and downstream SRC and PYK2.A, RT-qPCR assay showed that si-NgBR down-regulated ICAM-1 and downstream SRC and PYK2 mRNA expression. BC, Western blot analysis showed that si-NgBR down-regulated ICAM-1 and downstream SRC and PYK2 protein levels. The statistical values were analyzed by Student t-test. * p < 0.05 vs. the si-NC group; # p < 0.05 vs. the NgBR+ anti-NC group, n = 20.

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