AB236975

Anti-ICB1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ICB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human THEMIS2 aa 50-200.

대체 명칭 보기

C1orf38, ICB1, THEMIS2, Protein THEMIS2, Induced by contact to basement membrane 1 protein, Thymocyte-expressed molecule involved in selection protein 2, Protein ICB-1

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)

Paraffin-embedded human pancreatic cancer tissue stained for ICB1 using ab236975 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)

NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for ICB1 (green) using ab236975 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human THEMIS2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q5TEJ8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein ICB1 also known as Intermediate Complex B1 exhibits mechanical functions critical to cellular processes. With a molecular mass of approximately 50 kDa ICB1 is widely expressed in various tissues including the liver brain and muscles. This protein contributes to important mechanistic roles in cells influencing their structural and functional stability. Its distribution across different tissues indicates its involvement in diverse cellular functions.

Biological function summary

The role of ICB1 is linked to cellular signaling pathways and structural maintenance. It often forms part of a larger protein complex within the cytoplasm contributing to cytoskeletal integrity. Through its interactions ICB1 helps in the regulation of intracellular transport and communication. Its integration into multiprotein complexes highlights its importance in maintaining cellular architecture and communication networks.

Pathways

ICB1 participates in signaling cascades such as the MAPK and PI3K-Akt pathways. These pathways are essential for cell survival growth and differentiation. ICB1 interacts with proteins like Akt and ERK1/2 helping to mediate signals that influence cell fate decisions. Its role in these pathways highlights its importance in regulating cell proliferation and responses to external stimuli.

ICB1 has connections to neurodegenerative diseases and certain cancers. In Alzheimer's disease ICB1 is thought to influence the pathology through its interaction with tau proteins contributing to neurofibrillary tangle formation. In some types of cancer alterations in ICB1 expression affect cell cycle regulation promoting unrestrained growth. Understanding the involvement of ICB1 with proteins like tau and cyclin-dependent kinases in these contexts is important for therapeutic development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production (PubMed : 20644716). Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways (By similarity).

See full target information THEMIS2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death and differentiation 29:1834-1849 PubMed35301431

2022

Hypoxia-induced GLT8D1 promotes glioma stem cell maintenance by inhibiting CD133 degradation through N-linked glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Liu,Liping Jiang,Yulin Shi,Baiyang Liu,Yaomei He,Qiushuo Shen,Xiulin Jiang,Zhi Nie,Jun Pu,Cuiping Yang,Yongbin Chen

Oncogene 41:997-1010 PubMed34974522

2022

Novel function of THEMIS2 in the enhancement of cancer stemness and chemoresistance by releasing PTP1B from MET.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chieh Huang,Jia-Hau Yen,Yu-Wen Sung,Shiao-Lin Tung,Po-Ming Chen,Pei-Yi Chu,Ya-Chi Shih,Hsiang-Cheng Chi,Yi-Ching Huang,Shih-Jei Huang,Lu-Hai Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ICB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)

Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Cell Lines & Lysates

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise