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AB239606

Anti-IDH1 antibody [C12]

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(2 제품이 사용된 논문 )

Mouse Monoclonal IDH1 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IDH1 aa 50-350.

대체 명칭 보기

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human glioma tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human prostate tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human stomach tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-IDH1 antibody [C12] (AB239606)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody [C12] (AB239606)

All lanes:

Western blot - Anti-IDH1 antibody [C12] (ab239606) at 5 µg/mL

All lanes:

Recombinant human IDH1 protein

Predicted band size: 46 kDa

false

Western blot - Anti-IDH1 antibody [C12] (AB239606)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody [C12] (AB239606)

All lanes:

Western blot - Anti-IDH1 antibody [C12] (ab239606) at 3 µg/mL

Lane 1:

Human liver tissue lysate

Lane 2:

Pig liver tissue lysate

Secondary

All lanes:

HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 46 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

C12

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human IDH1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

O75874

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대체품

Primary Antibodies

AB212470

Anti-IDH1 antibody [IDH1/1152] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [IDH1/1152] - BSA and Azide free (AB212470)

  • 0
  • 0
Primary Antibodies

AB214803

Anti-IDH1 antibody [EPR12296] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (AB214803)

  • 0
  • 0
Primary Antibodies

AB242078

Anti-IDH1 antibody [EPR21002] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IDH1 antibody [EPR21002] - BSA and Azide free (AB242078)

  • 5
  • 1
Primary Antibodies

AB172964

Anti-IDH1 antibody [EPR12296]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (AB172964)

  • 5
  • 2
Primary Antibodies

AB230949

Anti-IDH1 antibody [EPR21002]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IDH1 antibody [EPR21002] (AB230949)

  • 0
  • 0

보완 제품

Assay Kits

AB288926

Human IDH1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IDH1 ELISA Kit (AB288926)

  • 0
  • 0
Primary Antibodies

AB131263

Anti-IDH2 antibody [EPR7577]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (AB131263)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 8:eabp8293 PubMed36525494

2022

In vivo characterization of glutamine metabolism identifies therapeutic targets in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akash K Kaushik,Amy Tarangelo,Lindsey K Boroughs,Mukundan Ragavan,Yuanyuan Zhang,Cheng-Yang Wu,Xiangyi Li,Kristen Ahumada,Jui-Chung Chiang,Vanina T Tcheuyap,Faeze Saatchi,Quyen N Do,Cissy Yong,Tracy Rosales,Christina Stevens,Aparna D Rao,Brandon Faubert,Panayotis Pachnis,Lauren G Zacharias,Hieu Vu,Feng Cai,Thomas P Mathews,Giannicola Genovese,Barbara S Slusher,Payal Kapur,Xiankai Sun,Matthew Merritt,James Brugarolas,Ralph J DeBerardinis

Journal of immunology (Baltimore, Md. : 1950) 158:1108-15 PubMed9013949

1997

Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

A M Oliver,F Martin,J F Kearney
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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