AB242130

Anti-IFFO1 antibody [EPR22711-61]

20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal IFFO1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

IFFO, IFFO1, Non-homologous end joining factor IFFO1, NHEJ factor IFFO1, Intermediate filament family orphan 1, Tumor antigen HOM-TES-103

3 이미지

Supplier Data

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

IFFO1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242130 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242130 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2 : ab242130 IP in HEK-293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242130 in HEK-293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 41 seconds

All lanes:

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (ab242130)

Observed band size: 75 kDa

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Lab

Western blot - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID : 31548606).

Negative control : HeLa (PMID : 31548606).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HDLM-2 (human hodgkin lymphoma) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 62 kDa

Observed band size: 75 kDa

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Collaborator

Western blot - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Data was kindly provided by an anonymous collaborator.

The antibody for IFFO2 is ab242131.

Lanes 1 - 4:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (<a href='/ko/products/primary-antibodies/iffo2-antibody-epr22712-35-ab242131'>ab242131</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

IFFO1 knockout HEK-293T whole cell lysate

Lane 3:

IFFO2 knockout HEK-293T whole cell lysate

Lane 4:

IFFO1/IFFO2 knockout HEK-293T whole cell lysate

Predicted band size: 57 kDa,62 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22711-61

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IFFO1 also known as Intermediate Filament Family Orphan 1 is a protein that plays a role in structural support in cells. It weighs approximately 63 kDa. IFFO1 is expressed in various tissues with a higher concentration in the heart and skeletal muscles and also found in the liver and testis. This target is involved in maintaining the integrity of the cytoskeleton by interacting with other cytoskeletal proteins.

Biological function summary

The protein supports cellular architecture and dynamics during cell division and differentiation. IFFO1 can interact with other intermediate filament proteins but researchers suggest it may also function independently. Though not very well-studied it contributes to the stability of cells' mechanical properties playing a part in maintaining cellular organization and providing resilience against mechanical stress.

Pathways

Its involvement in cytoskeletal organization connects it with the cytoskeletal remodeling pathway which is fundamental for cellular shape and stability. It associates with proteins like vimentin and desmin which are important in this pathway. These interactions underline its contribution to cell structure and mechanics connecting it to both cellular movement and morphological changes.

Evidence links IFFO1 to muscular dystrophy where muscle integrity and function diminish and perhaps to certain liver conditions. The protein interacts with key structural entities like desmin which is also implicated in these disorders. Dysregulation of IFFO1 or its interacting partners may contribute to the disruption of muscle and liver function leading to progressive muscle disorders or hepatic pathologies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed : 31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that relocalizes to the DSB sites in a XRCC4-dependent manner and promotes the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed : 31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed : 31548606).

See full target information IFFO1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:509 PubMed40634295

2025

Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanling Ye,Fan Shen,Maoli Yan,Conghui Zhan,Jiahua Lv,Mengqin Zou,Zhifeng Wang,Shaokai Ning,Yanfei Gao,Jingxian Wu,Wen Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFFO1 antibody [EPR22711-61]

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

Western blot - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

Western blot - Anti-IFFO1 antibody [EPR22711-61] (AB242130)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise