AB242131

Anti-IFFO2 antibody [EPR22712-35]

20ul selling size
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal IFFO2 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

대체 명칭 보기

Intermediate filament family orphan 2, IFFO2

3 이미지

Supplier Data

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

IFFO2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242131 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2 : ab242131 IP in HEK293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242131 in HEK-293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131)

Predicted band size: 57 kDa

Observed band size: 70 kDa

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Lab

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 2 : 15 seconds Lane 1, lane 3-5 : 59 seconds

All lanes:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HDLM-2 (human hodgkin lymphoma) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 70 kDa

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Collaborator

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Data was kindly provided by an anonymous collaborator.

The antibody for IFFO1 is ab242130.

Lanes 1 - 4:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (<a href='/ko/products/primary-antibodies/iffo1-antibody-epr22711-61-ab242130'>ab242130</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

IFFO1 knockout HEK-293T whole cell lysate

Lane 3:

IFFO2 knockout HEK-293T whole cell lysate

Lane 4:

IFFO1/IFFO2 knockout HEK-293T whole cell lysate

Predicted band size: 57 kDa,62 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22712-35

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IFFO2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 is a protein that typically weighs approximately 110 kDa. It localizes mainly in the cytoplasm and nucleus expressing in various tissues with higher presence in immune tissues and the brain. This protein participates in cellular signaling processes by interacting with other molecular complexes and proteins through its tetratricopeptide repeat domains. The role of IFFO2 in these regions suggests a part in coordinating cellular responses to external stimuli.

Biological function summary

IFFO2 contributes to immune modulation and cellular stress response. It associates with larger protein complexes aiding in the regulation of gene expression and protein stability. IFFO2 influences how cells react to viral infections through its interactions adapting cellular processes to enhance responsiveness to interferons. This dimension of functionality underlines its involvement in defensive mechanisms against pathogens showcasing its significance in immune regulation networks.

Pathways

The protein interacts within the interferon signaling pathway and plays a part in the regulation of apoptosis. IFFO2 relates to interferon regulatory factor 3 (IRF3) aiding in the enhancement of antiviral defenses. Additionally it may affect the expression of other important signaling molecules such as NF-kB showing its coordination role in controlling cell fate decisions. Its involvement in these pathways places it among the core regulators of immune and stress-response dynamics.

IFFO2 relates to neurodegenerative disorders and cancer. Its interaction with p53 protein points to its role in tumor suppression as it influences cell cycle arrest and apoptosis. Alterations in IFFO2 might contribute to pathological developments like Alzheimer's disease where disrupted immune function and protein aggregation are notable. Understanding IFFO2's regulatory impact could provide new insights into therapeutic strategies against these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information IFFO2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFFO2 antibody [EPR22712-35]

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise