AB118045

Anti-IFIT3/P60 antibody [OTI1G1]

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(3 제품이 사용된 논문 )

Mouse Monoclonal IFIT3/P60 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with African green monkey, Human, Transfected cell lysate samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT3.

대체 명칭 보기

CIG-49, IFI60, IFIT4, ISG60, IFIT3, Interferon-induced protein with tetratricopeptide repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60 kDa protein, Interferon-induced protein with tetratricopeptide repeats 4, Retinoic acid-induced gene G protein, IFI-60K, IFIT-4, P60, RIG-G

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.

Flow Cytometry (Intracellular) - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Supplier Data

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

All lanes:

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) extracts at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 35 µg

Lane 3:

SVT2 extracts at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) extracts at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 35 µg

Lane 7:

MDCK (Canine kidney cell line) extracts at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) extracts at 35 µg

Predicted band size: 56 kDa

false

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

All lanes:

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA at 5 µg

Predicted band size: 56 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1G1

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFIT3.

O14879

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant by affinity chromatography.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IFIT3 also known as P60 is a member of the IFIT (Interferon-Induced Proteins with Tetratricopeptide Repeats) family of proteins. With a molecular mass of approximately 60 kDa IFIT3 is part of the antiviral response system. This protein is expressed in various tissues and is highly inducible by type I interferons. It functions to inhibit viral replication by interacting with viral RNA. The structure of IFIT3 includes tetratricopeptide repeat motifs which facilitate its role in protein-protein interactions.

Biological function summary

IFIT3 plays a major role in the immune response against viral infections. The protein forms complexes with other IFIT family members like IFIT1 and IFIT2 to enhance antiviral efficacy. IFIT3 binds to foreign RNA lacking proper cap structures blocking viral protein synthesis. The protein's actions help strengthen the innate immune system and further control the spread of viral pathogens. IFIT3 also modulates cellular functions by engaging with signaling pathways that regulate inflammation and immune defense.

Pathways

IFIT3 integrates into important signaling networks such as the interferon-mediated signal transduction pathway and the JAK-STAT signaling pathway. In these pathways IFIT3 along with related proteins like IRF3 and ISG15 helps amplify immune responses after detecting viral threats. Its involvement in these pathways ensures that antiviral states within cells are quickly established reducing viral survival and dissemination.

IFIT3 has significant implications for infection and cancer outcomes. In viral infections such as hepatitis C IFIT3 expression levels correlate with effective antiviral activity and prognosis. In cancer aberrant IFIT3 expression can influence tumorigenesis and impact treatment responses. Collaborating with proteins such as STAT1 and p53 IFIT3 can affect pathways involved in cell proliferation and apoptosis linking immune responses to cancer progression and therapy resistance.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

See full target information IFIT3

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Clinical and translational medicine 14:e1800 PubMed39305055

2024

IFIT3 mediates TBK1 phosphorylation to promote activation of pDCs and exacerbate systemic sclerosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyang Huang,Yi Liu,Xia Rong,Yiheng Zhao,Dan Feng,Jun Wang,Wanhong Xing

Nature communications 10:2550 PubMed31186410

2019

Loss of 5-methylcytosine alters the biogenesis of vault-derived small RNAs to coordinate epidermal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahim A Sajini,Nila Roy Choudhury,Rebecca E Wagner,Susanne Bornelöv,Tommaso Selmi,Christos Spanos,Sabine Dietmann,Juri Rappsilber,Gracjan Michlewski,Michaela Frye

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFIT3/P60 antibody [OTI1G1]

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Flow Cytometry (Intracellular) - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise