AB113485

Anti-IFIT5 antibody

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(1 Publication)

Rabbit Polyclonal IFIT5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFIT5 aa 50-300.

대체 명칭 보기

ISG58, RI58, IFIT5, Interferon-induced protein with tetratricopeptide repeats 5, IFIT-5, Interferon-induced 58 kDa protein, Retinoic acid- and interferon-inducible 58 kDa protein, P58

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT5 antibody (AB113485)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT5 antibody (AB113485)

ab113485 at 1/100 dilution staining IFIT5 in Formalin-fixed, Paraffin-embedded Human fetal brain tissue by Immunohistochemistry.

Unknown

Western blot - Anti-IFIT5 antibody (AB113485)

All lanes:

Western blot - Anti-IFIT5 antibody (ab113485) at 1/1000 dilution

Lane 1:

Human fetal brain lysate

Lane 2:

Human fetal colon lysate

Predicted band size: 56 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IFIT5 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q13325

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab113485 is purified by a protein affinity column.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IFIT5 also known as Interferon-induced protein with tetratricopeptide repeats 5 is a protein with a molecular mass of approximately 56 kDa. This protein is expressed mainly in the cytoplasm where it functions by specifically binding to single-stranded RNAs lacking 2'-O-methylation at their 5'-cap structure. This recognition allows IFIT5 to distinguish self from non-self RNA an important element in immune defense mechanisms. Its ability to sense and bind to viral RNA makes it an important player in the host's innate immune response.

Biological function summary

IFIT5 is involved in antiviral defense and immune response regulation. It does not function as part of a multi-protein complex but interacts with various signaling molecules to amplify immune responses. By selectively binding to foreign RNA IFIT5 can inhibit viral replication and modulate type I interferon signaling pathways. It plays a significant role in restricting viral protein synthesis giving the host organism time to mount an adaptive immune response.

Pathways

IFIT5 is closely associated with the interferon signaling pathway and the innate immune response pathway. Through its interactions it affects the Jak-STAT signaling pathway which is important for the induction of antiviral states in cells. IFIT5 also has functional links with other proteins such as RIG-I an important sensor in the detection of viral RNAs. Together they provide a robust early immune defense by facilitating cytokine production and ensuring rapid response to infections.

IFIT5 has been linked to viral infections and certain inflammatory conditions. Its role in antiviral defense connects it to diseases such as hepatitis C where the protein's influence on interferon action can affect viral persistence and patient response to treatment. Additionally IFIT5 interaction with proteins like PKR moves it to the context of autoimmune disorders where overactive or dysregulated immune responses can lead to chronic inflammation and tissue damage.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed : 23317505, PubMed : 23774268, PubMed : 25092312). Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed : 23334420). Also recognizes and selectively binds AT-rich dsDNA (PubMed : 23774268). Additionally, as a mediator in innate immunity, positively regulates IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed : 26334375).

See full target information IFIT5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 30:34 PubMed38448811

2024

Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1.

Applications

Unspecified application

Species

Unspecified reactive species

Hengru Guo,Ying Xin,Saifei Wang,Xiaoning Zhang,Yanqi Ren,Bo Qiao,Hongjiang Li,Jing Wu,Xiao Hao,Lijun Xu,Yushan Yan,Haohao Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFIT5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT5 antibody (AB113485)

Western blot - Anti-IFIT5 antibody (AB113485)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise