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AB316830

Anti-IgD antibody [EPR26760-30] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal IgD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, IGHD

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Sandwich ELISA - Anti-IgD antibody [EPR26760-30] - BSA and Azide free (Capture) (AB316830)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IgD antibody [EPR26760-30] - BSA and Azide free (Capture) (AB316830)

Sandwich ELISA of ab316829 with the capture antibody ab316830 dilution at 2 ug/mL and detector antibody ab316831 dilution at 0.5 ug/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26760-30

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB157708

Human IgD ELISA Kit

Sandwich ELISA - Human IgD ELISA Kit (AB157708)

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Primary Antibodies

AB316998

Anti-beta 2 Defensin/BD-2 antibody [EPR26637-70] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-beta 2 Defensin/BD-2 antibody [EPR26637-70] - BSA and Azide free (Detector) (AB316998)

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Primary Antibodies

AB317278

Anti-DKK1 antibody [EPR22363-3] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-DKK1 antibody [EPR22363-3] - BSA and Azide free (Detector) (AB317278)

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  • 0
Primary Antibodies

AB316787

Anti-RUNX3 antibody [EPR24390-50] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-RUNX3 antibody [EPR24390-50] - BSA and Azide free (Detector) (AB316787)

  • 0
  • 0
Primary Antibodies

AB316825

Anti-NFAT4/NF-ATc3 antibody [EPR25800-1] - BSA and Azide free (Detector)

Recombinant|RabMAb|KO Validated

Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-1] - BSA and Azide free (Detector) (AB316825)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab316830 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IgD. The recommended pair for sandwich ELISA is:

Capture: ab316830, Human IgD Capture Antibody (unconjugated)

Detector: ab316831, Human IgD Detector Antibody (unconjugated)

The reference range value is 7.8-1000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.
Biological function summary

IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.

Pathways

IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.

IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD (PubMed : 10702483, PubMed : 11282392, PubMed : 8774350).
See full target information IGHD
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Product promise

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