AB235125

Anti-IgD antibody [IA6-2]

Recombinant
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Mouse Recombinant Monoclonal IgD antibody. Suitable for Flow Cyt, I-ELISA, WB and reacts with Human, Recombinant full length protein - Human samples.

대체 명칭 보기

Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, IGHD

3 이미지

Flow Cytometry - Anti-IgD antibody [IA6-2] (AB235125)

Flow Cyt

Unknown

Flow Cytometry - Anti-IgD antibody [IA6-2] (AB235125)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling IGHD with ab235125 at 1/500 dilution (2.064μg/ml) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab235125 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647. Gated on viable cells.

Indirect ELISA - Anti-IgD antibody [IA6-2] (AB235125)

I-ELISA

Lab

Indirect ELISA - Anti-IgD antibody [IA6-2] (AB235125)

ELISA - Anti-IGHD antibody [IA6-2] (ab235125).

ab235125 used at 0-4000 ng/ml.

Antigen (Human IGD) used at 1000 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

Lab

Western blot - Anti-IgD antibody [IA6-2] (AB235125)

This antibody recognize the heavy chain (~60 kDa) of IgD.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IgD antibody [IA6-2] (ab235125) at 1.032 µg/mL

Lane 1:

Human plasma lyaste at 10 µg

Lane 2:

Human serum lyaste at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 47 kDa

false

Exposure time: 8s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

IA6-2

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, I-ELISA

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2.064 µg/mL", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.032 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.032 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.032 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "16.645 µg/mL", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.

Biological function summary

IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.

Pathways

IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.

IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD (PubMed : 10702483, PubMed : 11282392, PubMed : 8774350).

See full target information IGHD

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgD antibody [IA6-2]

Flow Cytometry - Anti-IgD antibody [IA6-2] (AB235125)

Indirect ELISA - Anti-IgD antibody [IA6-2] (AB235125)

Western blot - Anti-IgD antibody [IA6-2] (AB235125)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise