AB124055

Anti-IgG antibody [R10Z8E9]

Recombinant
Lab Essentials
각 태그의 뜻

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(19 제품이 사용된 논문 )

Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse monoclonal antibody detecting IgG in Western Blot, ELISA. Suitable for Human.

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 이미지

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

I-ELISA

Lab

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Lab

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expected MW : 50 kDa.

All lanes:

Western blot - Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution

All lanes:

Human serum lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

Predicted band size: 36 kDa

false

Exposure time: 3min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

R10Z8E9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Human samples.

What is the molecular weight of IgG?
Anti-IgG [R10Z8E9] (ab124055) specifically detects a band for IgG (UniProt: P01857) at a molecular weight of 36kDa.

Other related products
We have a range of other formats of antibody clone [R10Z8E9] also available for your convenience: ab124055, Carrier free - ab256120

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information IgG

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제품이 사용된 논문 (19)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Open life sciences 20:20251126 PubMed40822968

2025

SATB2 promotes humeral fracture healing in rats by activating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liantao Liu,Shuai Rong,Xiaobin Zhou,Hao Li,Kepei Zhen,Chong Zheng,Kewei Li

Clinics (Sao Paulo, Brazil) 80:100653 PubMed40273496

2025

circPOLA2 promotes proliferation, invasion, migration, and epithelial-mesenchymal transition in breast cancer via the miR-1224-5p/HMGA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

XinYan Xu,Jie Li,RuiJuan Li,YanFang Tan,ZhiBing Lu

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Discover oncology 15:401 PubMed39225900

2024

Hsa_circYARS interacts with miR-29a-3p to up-regulate IREB2 and promote laryngeal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhao Guo,Yuxia Zhao,Naicai Guo,Meng Xu,Xiaolei Wang

The Kaohsiung journal of medical sciences 40:699-709 PubMed39031804

2024

circUQCRC2 promotes asthma progression in children by activating the VEGFA/NF-κB pathway by targeting miR-381-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Yang,Shu-Xiang Sui,Qing-Hua Zheng,Min Wang

Discover oncology 15:165 PubMed38748048

2024

circ_0006789 promotes cervical cancer development via the miR-615-5p/HSF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhou,Weiwei Song,Meisong Lu

Molecular systems biology 20:719-740 PubMed38580884

2024

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Applications

Unspecified application

Species

Unspecified reactive species

YiQing Lü,Tiffany Cho,Saptaparna Mukherjee,Carmen Florencia Suarez,Nicolas S Gonzalez-Foutel,Ahmad Malik,Sebastien Martinez,Dzana Dervovic,Robin Hyunseo Oh,Ellen Langille,Khalid N Al-Zahrani,Lisa Hoeg,Zhen Yuan Lin,Ricky Tsai,Geraldine Mbamalu,Varda Rotter,Patricia Ashton-Prolla,Jason Moffat,Lucia Beatriz Chemes,Anne-Claude Gingras,Moshe Oren,Daniel Durocher,Daniel Schramek

Cartilage 16:486-494 PubMed38366389

2024

HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanchi Huang,Wenjie Pan,Huanli Bao,Xiangxiang Sun,Chao Xu,Jianbing Ma

Molecular metabolism 65:101582 PubMed36030035

2022

RIPK1 and RIPK3 regulate TNFα-induced β-cell death in concert with caspase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Contreras,Noyonika Mukherjee,Renato C S Branco,Li Lin,Meghan F Hogan,Erica P Cai,Andrew A Oberst,Steven E Kahn,Andrew T Templin

Histology and histopathology 37:899-907 PubMed35451495

2022

AADAC promotes therapeutic activity of cisplatin and imatinib against ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haijing Wang,Disong Wang,Tingting Gu,Mengjiao Zhu,Ling Cheng,Wentao Dai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG antibody [R10Z8E9]

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (19)

Product promise